Recognizes the ~35 kDa E2, E3, and E4 isoforms of apolipoprotein E in human serum and brain.
Synonyms: Anti-ApoE
Application: ELISA (see comments)Immunoblotting (1:100-1:1000)Immunoprecipitation (see comments)Frozen Sections (1:100-1:10,000)Paraffin Sections (1:100-1:10,000, formic acid or heat pre-treatment required)
Legal Information: CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes: Recognizes the ~35 kDa E2, E3, and E4 isoforms of apolipoprotein E in human serum, but multiple bands are seen when used with brain tissue samples. Staining of formalin-fixed paraffin-embedded tissues may be enhanced by the pre-treatment of tissue with 70% formic acid for 10-30 min at room temperature or by heat pre-treatment using citrate buffer, pH 6.0. This antibody has also been reported to work for immunoprecipitation and ELISA. Antibody should be titrated for optimal results in individual systems.