Anti-Apg8 purified polyclonal antibody reacts with yeast APG8 by western blot and ELISA. This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or their conjugates). Other intrinsic bands are readily detectable in yeast lysates at lower antibody dilutions. For immunoblotting a 14 kDa band corresponding to yeast Apg8 is detected. Most yeast cell lysates can be used as a positive control without induction or stimulation. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.