Anti-Apaf1 Antibody has been tested for use in ELISA, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Paraformaldehyde fixation is recommended for immunohistochemical staining. Expect a band at ~130 kDa in size corresponding to Apaf1 by western blotting in the appropriate cell lysate or extract. K562 cells can be used as a positive control.