Monoclonal Anti- a Actinin (mouse IgM isotype) is derived from the hybridoma BM-72.5 produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with a cytoskeletal fraction of bovine mammary gland epithelium (BMGE) cultured cells. a-Actinin is an actin binding protein present in both muscle and non-muscle cells. It has a molecular weight of 100,000 daltons and forms dimers in solution. In normal skeletal muscle, a-actinin is associated with the z-discs that define muscle sarcomeres. In smooth muscle, a-actinin has been detected in dense bodies and plaques characteristic of the tissue.
Synonyms: Alpha-actinin cytoskeletal isoform; Alpha-actinin-1; F-actin cross-linking protein; Non-muscle alpha-actinin-1
Storage: -20C
Application: Anti-alpha Actinin antibody may be used in various immunochemical techniques including Immunoblot (~100 kDa), Immunohistochemistry and Immunofluorescence. Monoclonal Anti-alpha-Actinin may be used for immunofluorescent localization of alpha-actinin in cultured cells and tissues, for immunofluorescent labeling of skeletal muscle (normal and pathological) in order to detect its organizational state, studies of membrane anchorage sites and immunochemical identification of alpha-actinin by immunoblotting analysis.
Biochem Physiol Actions: a-actinin modulates muscle contraction and has a role in cytoskeletal organisation. a-actinin acts as an actin crosslinker and scaffold. Mutations in this gene has been linked to heterogeneous hypertrophic cardiomyopathy and juvenile onset atrial fibrillation.