Anti-alkaline phosphatase has been assayed against 1.0 ug of Alkaline Phosphatase [E.coli] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:3,500 to 1:15,000 of the reconstitution concentration is suggested for this product.