ALKALINE PHOSPHATASE Antibody has been assayed against 1.0 ug of Alkaline Phosphatase [E.coli] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:25,000 to 1:125,000 of the reconstitution concentration is suggested for this product. A 1:10,000 dilution has been reported to be useful for the detection by immunoblot of an integral membrane protein from a gram-positive organism expressed as a LacZ/PhoA chimeric derivative in E.coli. The researcher should determine optimal titers for other applications.