Anti-AKR1C3 antibody, Mouse monoclonal (humana - hydroxysteroid dehydrogenase, type 2,3; 17ß- hydroxysteroid dehydrogenase, type 5) (mouse IgG1 isotype) is derived from the hybridoma NP6.G6. A6 produced by the fusion of mouse myeloma cells (SP20 cells) and splenocytes from BALB/c mice immunized with human AKR1C3 protein. Oxidoreduction of natural and foreign substrates is performed by three enzymes superfamilies, one of them being the AKRs (aldo-keto reductases) family. This family contains 114 proteins that are expressed in prokaryotes and eukaryotes and is divided into 14 subfamilies (AKR1-AKR14). The AKR1 family is the largest among the different fourteen families and contains the aldose reductases, aldehyde reductases, hydroxysteroid dehydrogenases, and steroid 5 ß-reductases. AKR1Cs are expressed in many tissues.
MDL Number: MFCD06798693
Storage: -20C
Application: Mouse monoclonal clone NP6.G6.A6 anti-AKR1C3 antibody used to tag aldo-keto reductase 1C3 for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of aldo-keto reductase 1C3 in the regulation of the androgen 5alpha-dihydrotestosterone (DHT) and 3alpha-diol compound levels in vivo.
Biochem Physiol Actions: In humans AKR1C3 (aldo-keto reductase family 1 member C3) including AKR1C1,2 and 4, catalyze the reduction of the androgen 5a-dihydrotestosterone (DHT) into inactive 3ß or 3a-androstane diol (3a/ß-Diol). AKR1C1-4 isoforms also display 3a[17ß]-hydroxysteroid oxidase activity using 3a-Diol as a substrate, in vitro. In non-small cell lung carcinoma, AKR1Cs expression is dramatically increased. Due to their product profile and discrete tissue localization, they may regulate the level of active androgens, estrogens, and progestins in target tissues.