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MilliporeSigma

Anti-Active-beta-Catenin Antibody, clone 8E7, Alexa Fluor(R) 555 clone 8E7, from mouse, ALEXA FLUOR(R) 555

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Catenin beta-1 (UniProt P35222; also known as beta-Catenin) is encoded by the CTNNB1 (also known as CTNNB, MRD19) gene (ORF OK/SW-cl.35, PRO2286; Gene ID 1499) in human. beta-Catenin is a multifunctional protein involved in the regulation of cell-cell adhesion and in the control of the Wnt signaling pathway. beta-Catenin contains in its central region 12 armadillo repeats that mediate its interaction with a variety of proteins including axin, adenomatous polyposis coli (APC), and T cell factor (TCF) family transcription factors. The C-terminal domain (CTD) of beta-catenin is implicated in transcriptional functions, while the N-terminal domain (NTD) contains several posttranslational modification (ubiquitination, acetylation, and phosphorylation) sites, including the Ser33/Ser37/Thr41 residues, important for beta-catenin stability regulation. In the absence of Wnt pathway activation, beta-catenin is retained in the cytoplasm in a "destruction complex" with Axin, APC, and GSK3beta, where it is constitutively destined for proteasomal degradation as a result of constant phosphorylation by GSK3beta. Activation of the Wnt pathway results in stabilization and nuclear translocation of beta-catenin, resulting in beta-catenin target genes transcription. In addition to GSK3beta, PKCalpha is also reported to phosphorylate the Ser33/37/Thr41 residues of beta-catenin, and beta-catenin Ser45 is a known phosphorylation site for CK1alpha. In addition, HDAC6 and p300/CBP-associated factor (PCAF) regulate beta-catenin Lys49 acetylation.

Synonyms: Catenin beta-1, Beta-catenin, Active-beta-Catenin

Application: This Anti-Active-beta-Catenin Antibody, clone 8E7, Alexa Fluor(R) 555 is validated for use in Immunocytochemistry, Immunohistochemistry for the detection of Active-beta-Catenin.

Legal Information: ALEXA FLUOR is a registered trademark of Life Technologies

Other Notes: Concentration: Please refer to lot specific datasheet.

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Product Detail
Thomas No.
CHM02Z979
Mfr. No.
05-665-AF555
Description
05-665-AF555, Anti-Active-beta-Catenin Antibody, clone 8E7, Alexa Fluor(R) 555 clone 8E7, from mouse, ALEXA FLUOR(R) 555
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