Anti-ABCB5 (N-term) antibody produced in rabbit, saturated ammonium sulfate (SAS) precipitated, buffered aqueous solution

Synonyms: Anti-ABCB5; Anti-ABCB5 P-gp; Anti-ATP-binding cassette sub-family B member 5; Anti-P-glycoprotein ABCB5

Storage: -20C

UNSPSC Code: 12352203

General description: ABCB5 (ATP binding cassette subfamily B member 5) gene is located in human chromosome 7p21.1. The encoded protein belongs to the subfamily B multidrug resistance and ATP-binding cassette superfamily of active transporters. The encoded protein have two identical halves, each contains a hydrophobic region with six transmembrane segments and a nucleotide-binding region. Expression of this gene is observed in prostate and testis and in human melanoma tumor-initiating cells.

General description: ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules.

Application: Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Flow cytometry/Cell sorting (1 paper)

Biochem/physiol Actions: ABCB5 (ATP binding cassette subfamily B member 5) is a cell surface marker for melanoma-initiating cells, on cellular metabolism. ABCB5 significantly regulates glycolysis, glutaminolysis and phospholipid metabolism. It induces stem cell differentiation, stem cell immortality and anti-apoptotic activity. The gene is upregulated in many cancer subtypes, including breast cancer. Recent studies reveal that ABCB5 is a novel marker for limbal stem cell and is needed for corneal development and repair. ABCB5 shows resistance against taxanes and anthracyclines, the anti-cancer drugs.

Immunogen: ABCB5 (NP_848654, 3-35)This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human ABCB5.

Physical form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.