AnteoBind Micro was developed for binding antibodies and other proteins to magnetic particles greater than 1 mum in size. It forms strong multi-valent interactions with almost any electron donating groups including carboxylic acids. Once proteins are added, AnteoBind activated particles rapidly form very strong but gentle binding interactions.AnteoBind Micro has been used to stably activate many particles. Examples include: M-270 Dynabeads(R), Bangs ProMag(R), JSR MS300 Carboxyl and Estapor(R) M1-200/20.AnteoBind Micro has many advantages over existing methods such as EDC/NHS chemistry or alternative "pre-activated" methods such as Epoxy or Tosyl. AnteoBind is water based and replaces the dry chemicals you would use with the EDC method. Using AnteoBind instead of NHS/EDC chemistry can save up to 3-4 hours of lab time. Experiments have shown increased stability and functionality for antibodies and a decreased background. AnteoBind improves reproducibility between experiments by providing uniformity between surfaces. For particles from 0.5 mum to 1 mum the recommended AnteoBind product is AnteoBind Sub-Micron.
Application: Activation and coupling of particles > 1 mum in diameter
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