Annexin V fluorescent conjugates can be used to detect apoptotic cells. The human anticoagulant Annexin V is a 35-36 kDa calcium-dependent phospholipid-binding protein with high affinity for phosphatidylserine (PS). In normal viable cells, PS is located on the inner leaflet of the cytoplasmic membrane. In apoptotic cells, however, PS is translocated from the inner to the outer leaflet of the plasma membrane, where it can be detected by fluorescently labeled Annexin V. CF® Dye Annexin V Conjugates are provided at 50 ug/mL in buffer with azide as a preservative. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Blue fluorescent CF®405M dye has excitation/emission at 408/452 nm.
29009R-5ug Annexin V is a blue fluorescent conjugate (excitation/emission 408/452 nm) that can be used to stain phosphatidylserine on the surface of apoptotic cells. This Annexin V conjugate is supplied lyophilized and azide-free, compatible with real-time imaging of live cells. Unit size 5 ug.