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Jackson Immunoresearch

Alkaline Phosphatase Streptavidin

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Alkaline Phosphatase Streptavidin

Steptavidin, a bacterial protein isolated from Streptomyces avidinii, is similar to egg-white avidin in its ability to bind biotin, and has been used as a replacement for egg-white avidin because of its more favorable chemical properties.

Conjugates of streptavidin are recommended for use with Biotin-SP-conjugated antibodies and Biotin-SP-conjugated ChromPure proteins.

Conjugate: Alkaline Phosphatase

Alkaline phosphatase (from calf intestine) conjugates are prepared by a modified method of Avremeas et al., Scand. J. Immunol. 1978. 8 (Supple. 7), 7. Resulting conjugates contain heterogeneous, high molecular weight complexes. They are sensitive reagents for solid-phase immunoassays such as ELISA and Western blotting. Although alkaline phosphatase conjugates are sometimes used for immunohistochemistry, penetration into whole mount tissues may be limited by their large sizes.

Physical StateSterile-filtered liquid 
StorageStore at 2-8°C. Prepare working dilution fresh each day. For extended storage, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Note: adding glycerol reduces the stated protein concentration and dilution range by one-half. 
Expiration dateOne year from the date of receipt. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
Buffer0.01M Tris-HCl, 0.25M NaCl, pH 8.0  
Stabilizer15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) 
Preservative0.05% Sodium Azide 
Suggested Working Concentration or Dilution Range
ELISA1-2 µg / ml
Western Blot0.1-1 µg / ml
Histo-/Cyto-Chemistry1-2 µg / ml
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

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Thomas No.
C840F76
Mfr. No.
016-050-084
Description
Alkaline Phosphatase Streptavidin, 1 mg
list price/quantitytotal
$0.00
$0.00 (0 Items)
Product C840F76 is restricted and can only be purchased by customers with a web profile linked to a Thomas Business Account - Click here to login or create your web profile. If you do not have a Thomas Business Account, please Contact Us and someone will respond with details on how to apply for a business account with us.

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