Synonym(s): BM blue; Sure Blue TMB; TMB; TMB Blotting Plus; TMB substrate
Formula: [-C6H2(CH3)2-4-NH2]2
Formula Weight: 240.34
CAS No.: 54827-17-7
Purity: ≥99%
Application: 3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin,3,4, assay of peroxidases.6,7,
Storage: 2-8°C
Packaging: 1, 5, 25 g in glass bottle
Beilstein Registry Number: 2808541
EC No.: 259-364-6
Melting Point: 168-171°C(lit.)
Empirical Formula (Hill Notation): C8H12O2