Synonyms: beta-Mercaptoethanol; 2-Hydroxyethylmercaptan; BME; Thioethylene glycol
CAS Number: 60-24-2
Formula: C2H6OS
Formula Weight: 78.13
Linear Formula: HSCH2CH2OH
MDL No.: MFCD00004890
Purity: >=99.0%
Boiling Point: 157 C(lit.)
Density: 1.114 g/mL at 25 C(lit.)
Refractive Index: n20/D 1.500(lit.)
Storage: 2-8C
Beilstein Registry Number: 773648
EC Number: 200-464-6
UNSPSC Code: 12352100
General description: 2-mercaptoethanol is a thiol compound, commonly used as a reducing agent in organic reactions.
Application: BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.
Application: Widely used for retarding oxidation of biological compounds in solution.
RTECS: KL5600000
RIDADR: UN 2966 6.1 / PGII
WGK Germany: 3
Flash Point(F): 165.2 F
Flash Point(C): 74 C
R Codes: 23/24/25-38-41-43-48/22-50/53
S Codes: 26-36/37/39-45-60-61
IOD Codes: T,N
Symbol: GHS05, GHS06, GHS08, GHS09
Signal Word: Danger
Hazard Statements: H301 + H331-H310-H315-H317-H318-H373-H410
Precautionary statements: P260-P262-P273-P280-P301 + P310 + P330-P302 + P352 + P310-P305 + P351 + P338 + P310-P391-P403 + P233