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Jackson Immunoresearch

AffiniPure Goat Anti-Mouse IgM, µ Chain Specific

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Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the heavy chain of mouse IgM but not with mouse IgG or the light chains of mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, bovine and horse serum proteins, but it may cross-react with IgM from other species.

Target: Mouse

Host: Goat

Antibody Format: Whole IgG

Specificity: IgM, µ Chain Specific

Minimal Cross Reactivity: Human, Bovine, Horse Serum Proteins

Conjugate: Unconjugated

Clonality: Polyclonal

Physical StateSterile-filtered liquid 
StorageStore at 2-8°C under sterile conditions. Prepare working dilution fresh each day. 
Expiration dateone year from date of receipt. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
PurityThe antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. 
Buffer0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 
Stabilizer
PreservativeNone
Suggested Working Concentration or Dilution Range
ELISA:- 10-20 µg / ml 
Western Blot:- 10-20 µg / ml
Histo-/Cyto-chemistry:- 10-20 µg / ml
Flow Cytometry:- 10-20 µg / ml 
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

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Product Detail
Thomas No.
C840T27
Mfr. No.
115-005-075
Description
AffiniPure Goat Anti-Mouse IgM, µ Chain Specific, 1.0 mg
list price/quantitytotal
$0.00
$0.00 (0 Items)
Product C840T27 is restricted and can only be purchased by customers with a web profile linked to a Thomas Business Account - Click here to login or create your web profile. If you do not have a Thomas Business Account, please Contact Us and someone will respond with details on how to apply for a business account with us.

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