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Jackson Immunoresearch

AffiniPure Goat Anti-Mouse IgG, Light Chain Specific for Western blotting after IP

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AffiniPure Goat Anti-Mouse IgG, Light Chain Specific for Western blotting after IP

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Based on antigen-binding assay, Western blotting, and/or ELISA, the antibody reacts with the light chains on mouse IgG and with those common to other mouse immunoglobulins. Reaction is primarily with kappa light chains. The antibody does not react with the heavy chain of mouse IgG. The antibody has been tested by ELISA to ensure minimal cross-reaction with bovine, goat, horse, human, rabbit, rat and sheep immunoglobulins, but it may cross-react with immunoglobulins from other species.

Target: Mouse

Host: Goat

Antibody Format: Whole IgG

Specificity: IgG, Light Chain Specific for Western blotting after IP

Minimal Cross Reactivity: Bovine, Goat, Horse, Human, Rabbit, Rat, Sheep Immunoglobulins

Conjugate: Unconjugated

Clonality: Polyclonal

Physical StateSterile-filtered liquid 
StorageStore at 2-8°C under sterile conditions. Prepare working dilution fresh each day. 
Expiration dateone year from date of receipt. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
PurityThe antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. 
Buffer0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 
Stabilizer—
PreservativeNone
Suggested Working Concentration or Dilution Range
ELISA:- 10-20 µg / ml 
Western Blot:- 10-20 µg / ml
Histo-/Cyto-chemistry:- 10-20 µg / ml
Flow Cytometry:- 10-20 µg / ml 
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

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Product Detail
Thomas No.
C840T31
Mfr. No.
115-005-174
Description
AffiniPure Goat Anti-Mouse IgG, Light Chain Specific for Western blotting after IP, 1.0 mg
list price/quantitytotal
$0.00
$0.00 (0 Items)
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