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Jackson Immunoresearch

AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgM, µ Chain Specific

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AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgM, µ Chain Specific

F(ab')2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')2 fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G.

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the heavy chain of mouse IgM but not with mouse IgG or the light chains of mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with IgM from other species.

Target: Mouse

Host: Donkey

Antibody Format: F(ab')2 Fragment

Specificity: IgM, µ Chain Specific

Conjugate: Unconjugated

Clonality: Polyclonal

This product is for IN VITRO RESEARCH USE ONLY. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.

Physical StateSterile-filtered liquid 
StorageStore at 2-8°C under sterile conditions. Prepare working dilution fresh each day. 
Expiration dateOne year from date of receipt. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
PurityThe antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed. 
Buffer0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6  
PreservativeNone 
Suggested Working Concentration or Dilution Range
ELISA10-20 µg / ml 
Western Blot10-20 µg / ml 
Histo-/Cyto-chemistry10-20 µg / ml 
Flow Cytometry10-20 µg / ml 
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

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Product Detail
Thomas No.
C839P02
Mfr. No.
715-006-020
Description
AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgM, µ Chain Specific, 1 mg
list price/quantitytotal
$0.00
$0.00 (0 Items)
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