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Jackson Immunoresearch

AffiniPure Fab Fragment Goat Anti-Human IgG (H+L)

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AffiniPure Fab Fragment Goat Anti-Human IgG (H+L)

Fab fragment antibodies are generated by papain digestion of whole IgG antibodies to remove the entire Fc portion, including the hinge region. These antibodies are monovalent, containing only a single antigen binding site. The molecular weight of Fab fragments is about 50 kDa.

Based on antigen-binding assay and/or ELISA, the antibody reacts with whole molecule human IgG. It also reacts with the light chains of other human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species

Target: Human

Host: Goat

Antibody Format: Fab Fragment

Specificity: IgG (H+L)

Conjugate: Unconjugated

Clonality: Polyclonal

This product is for IN VITRO RESEARCH USE ONLY. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.

Physical StateSterile-filtered liquid
StorageStore at 2-8°C under sterile conditions. Prepare working dilution fresh each day.
Expiration dateOne year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
PurityThe antibody was purified from antisera by a combination of papain digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
Buffer0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6  
PreservativeNone
Suggested Working Concentration or Dilution Range
ELISA10-20 µg / ml
Western Blot10-20 µg / ml
Histo-/Cyto-Chemistry10-20 µg / ml
Flow Cytometry10-20 µg / ml
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

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Product Detail
Thomas No.
C840J44
Mfr. No.
109-007-003
Description
AffiniPure Fab Fragment Goat Anti-Human IgG (H+L), 1 mg
list price/quantitytotal
$0.00
$0.00 (0 Items)
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