Agilent Endoglycosidases (formerly ProZyme) release oligosaccharides from glycoproteins and some other substrates. These enzymes are useful for deglycosylating glycoproteins prior to analysis, or for releasing N-glycans for labeling and analysis. Useful in N-glycan analysis, Agilent offers PNGase F, an asparagine amidase that releases intact N-glycans. PNGase F cleaves between the innermost GlcNac of the N-glycan and the asparagine (Asn) residue of the peptide to which the N-glycan is linked. AdvanceBio Endo F2, recombinant (formerly ProZyme). Enzyme cleaves within the chitobiose (GlcNAc-GlcNAc) core of high mannose and biantennary complex type N-glycans. Includes 5X reaction buffer, which when diluted gives 200 mM sodium acetate pH 4.5.
This product is only available to U.S. Domestic Customers.
For research use only, not to be used in diagnostic procedures.