AnteoTech identified the following problems that researchers are having with their multiplex assays:
•Binding difficult antibodies and antigens to microspheres
•Complex protocols for inexperienced users
•Lengthy microsphere and protein preparation times prior to protein coupling
•Reproducibility between batches of protein coupled microspheres
•Limited storage of ready-to-couple microspheresFrom these findings, they developed the AnteoBind(TM) Activation Kit For Multiplex Microspheres that is for use with the Luminex xMAP(R) system and contains the patented AnteoBind Activation Reagent. With this Activation Kit, you can couple multiple antibodies without extra optimization for each, saving assay development time and reducing the barriers of assay transfer between diverse platforms.AnteoBind is water based and replaces the dry chemicals you would use with the EDC method. Using AnteoBind instead of NHS/EDC chemistry can save up to 3-4 hours of lab time. Experiments have shown increased stability and functionality for antibodies and a decreased background. AnteoBind improves reproducibility between experiments by providing uniformity between surfaces. You can store your AnteoBind activated microspheres for up to 1 year.POSTER: A Novel User Friendly Chemistry to Couple Antibodies and Challenging Proteins to Luminex MagPlex Microspheres
Storage: 2-8C
Application: Activation and coupling of Luminex Microspheres for Multiplex Immunoassays
Legal Information: xMAP is a registered trademark of Luminex Corp