Synonyms: AChE, Acetylcholine acetylhydrolase, Cholinesterase, Acetyl
CAS No.: 9000-81-1
Analysis Note: The activity obtained using acetylcholine as substrate is 30-100 times that obtained with butyrylcholine, using acetylcholinesterase from electric eel.
Biochem/physiol Actions: Major degradative enzyme for acetylcholine in vivo. Converts acetylcholine + H2O to choline + acetic acid.
Caution: For dilute solutions (<1 mg l), add 1 mg l bsa to stabilize. solutions containing at least 1 mg protein l in dilute neutral phosphate buffer are stable at least 6 months refrigerated.
Unit Definition: One unit will hydrolyze 1.0 µmole of acetylcholine to choline and acetate per min at pH 8.0 at 37 °C.
Physical Form: Lyophilized powder containing Tris buffer salts
General Description:
Molecular Weight: 280 kDa
Isoelectric Point: 5.5
Extinction Coefficient: E1% = 18.0 (280 nm)
Acetylcholinesterase from Electrophorus electricus is a tetramer composed of 4 equal subunits of 70 kDa each. Each subunit contains one active site. The enzyme is a glycoprotein containing hexosamines.