Acylation, an alternative to silylation, enables the conversion of compounds that comprise of active hydrogens (-OH, -SH and -NH) into esters, thioesters, and amides by the action of a carboxylic acid or derivative. The carbonyl group adjacent to the halogenated carbons favors the improvement of electron capture detector (ECD) response. Acylation has several benefits:
•It increases the stability of compounds by protecting unstable groups.
•It may render volatility on substances like carbohydrates or amino acids, that have several polar groups that they are non-volatile and generally decompose on heating.
•It allows the separations not possible with underivatized compounds.
•Compounds are detectable at very low levels with an ECD.Acetic anhydride is a reagent utilized to form fragmentation-directing derivatives for GC/MS analysis.
Molecular Formula: C4H6O3
Molecular Weight: 102.09
Linear Structural Formula: (CH3CO)2O
MDL Number: MFCD00008705
Purity: >=99.0% (GC); >=99.0%
Melting Point: -73 C (lit.)
Density: 1.08 g/mL (lit.)
Refractive Index: n20/D 1.390 (lit.)
Application: Suitable for the derivatization of acetyl, acetaminophen, alcohols, aldoses and partially methylated aldoses, aldosterone, amines, amino acids, amino sugars (in polysaccharides), amino sugar methyl glycosides, 2-amino-2-deoxyhexoses, arginyl peptides, aryl sulfate esters, bile acids, carbamates, carbohydrates, cerebrosides, cystine and cysteine (in peptides), desmethyldoxepin, 3,4-dihydroxyphenylglycol, estrogens, glycitols, hexopyranosides, hexosamines, hydroxamic acids, hydroxyamines, n-hydroxycarbamates, hydroxy steroids, inositols, 4-methoxy and 3,4-dimethoxyphenethylamine, methyl ester, methyl(methyl O-methyl-a-D-glucopyranosid)uronates, nucleosides, nucleoside bases and sugars, partially methylated pentose and hexoes, peptides, phenolalkylamines, phenylethylamines, polysaccharide constituents, postaglandins A,B and E, serotonin, sialic acids, sugars, natural sugars and tryptamine.
Legal Information: LiChropur is a trademark of Merck KGaA, Darmstadt, Germany
Other Notes: Reagent for acetic anhydride, acetyl, n-acetyl, n-acetyl-n, o-acetyl, acetate, aldononitrile acetate, aminoglycitol acetate, aryl acetate, permethyl acetyl, methyl ester, trifluoroacetyl, bis-trimethylsilyl, trimethylsilyl polyamino alcohol, and O-trimethylsilyl (TMS).