AccuGENE™ TBE (Tris hydroxymethyl aminomethane) is made from Tris, EDTA and Boric acid. The addition of borate makes TBA a better conductive medium than TAE so it less prone to overheating and better for longer runs. Borate does inhibit some enzymatic reactions, so it is not often used if the DNA/RNA will be extracted for downstream applications. AccuGENE™ Electrophoresis Buffers are formulated to match Lonza's Reliant™, Latitude™,and PAGEr™ Precast Gels, Long Ranger™ Gel Solution sequencing products, and the MDE™ Gel Solution product line. AccuGENE™ Buffers for DNA, RNA, and protein electrophoresis are prepared with high quality reagents and use 18 megOhm water. Products are filtered using a 0.45-micron filter.