7a-Hydroxy-4-cholesten-3-one (C4) is known as a marker for the cholesterol 7a-hydroxylase activity. It plays an important role in bile acid metabolism.
Synonyms: 7alpha-Hydroxycholest-4-en-3-one
Molecular Formula: C27H44O2
Molecular Weight: 400.64
Linear Structural Formula: C27H44O2
MDL Number: MFCD00870345
Purity: >=95.0% (HPLC)
Storage: -20C
Application: C4 may be used as a reference standard for the determination of C4 in:
•Human serum by ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) with electrospray ionization source (ESI) as well as LC-MS.
•Rat and monkey plasma by LC-ESI-MS/MS working on multiple reaction monitoring (MRM) mode of detection.
•Peripheral blood plasma by solid-phase extraction (SPE) and HPLC.
Biochem Physiol Actions: 7a-Hydroxycholestene-3-one, a metabolite in bile acid synthesis, is derived from 7a-hydroxycholesterol and can be further metabolized to 7a,12a,-dihydroxycholest-4-en-3-one. Analysis of 7a-Hydroxycholestene-3-one (HCO) in serum may serve as a novel, simple, and sensitive method for the detection of bile acid malabsorption in patients with chronic diarrhea of unknown origin.