Molecular Formula: C10H7N3O2
Molecular Weight: 201.18
Linear Structural Formula: C10H7N3O2
MDL Number: MFCD16652405
Purity: 97%
Melting Point: 117.8 C
Application: 7-Azido-4-methylcoumarin (AzMC) is a fluorogenic probe useful for the detection of hydrogen sulfide (H2S). The aromatic azide moiety is selectively reduced in the presence of H2S, producing the fluorescent 7-amino-4-methylcoumarin (AMC) with a concomitant increase in fluorescence with ?ex = 365nm and ?em = 450nm. The probe can be used both to monitor enzymatic production of H2S in vitro and also to visualize H2S in cells. The probe is highly sensitive and selective for H2S, providing a linear detection range in vitro between 200nm - 100µM H2S with 10µM probe, and reacting selectively with H2S in the presence of mM concentrations of cysteine, homocysteine, glutathionine and many other common biological analytes. Caution: Use of this product with DTT, TCEP and/or biological thiols >25mM should be avoided for maximum efficiency. AzMC is readily soluble in DMSO.