5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt BioReagent, for molecular biology, powder, =99%

Synonym: BCIP® p-toluidine salt, X-phosphate p-toluidine salt

CAS Number: 6578-06-9

Empirical Formula (Hill Notation): C₈H₆BrClNO₄P · C₇H₉N

Molecular Weight: 433.62

EC Number: 229-506-1

MDL Number: MFCD00040636

PubChem Substance ID: 24891823

Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.

Substrates
Histochemical substrate for alkaline phosphatase.

Application
For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.
BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.

Legal Information
BCIP is a registered trademark of Sigma-Aldrich Co. LLC