The lung epithelium undergoes a complex series of endoderm–mesoderm-mediated signaling events to generate a complex arborized network of conducting airway (bronchi, bronchioles) and gas-exchanging units (alveoli). Lung epithelium differentiation stages include: anterior/posterior and dorsal/ventral patterning, lung specification, lung budding, branching morphogenesis, and maturation. The 3dGRO Lung Organoid Maturation Medium is a chemically defined/serum-free ready-to-use media used to differentiate lung bud organoids (LBOs) into mature lung organoids that structurally resemble the in vivo branching airway and early alveolar structures. Large numbers of mature lung organoids may be generated that express appropriate markers indicative of multiple cell types found in the mature lung and airways including: SFTPB and SFTPC, MUC5AC, EpCAM, Sox9, Nkx2.1, acetyl-a-tubulin and Vimentin. Additionally, the mature lung organoids express ACE2 and TMPRSS2 for SARS-CoV2 viral research. The 3dGRO Lung Organoid Maturation Medium is a component of the 3dGRO Human Lung Organoid Culture System which can be used to differentiate human iPS cells into mature lung organoids that structurally resemble the in vivo branching airway and early alveolar structures.The 3dGRO(TM) Human Lung Organoid Culture System includes (all sold separately): • Definitive Endoderm Induction Medium (SCM302)• Anterior Foregut Endoderm Induction Medium I (SCM305)• Anterior Foregut Endoderm Induction Medium II (SCM306)• 3dGRO(TM) Lung Organoid Branching Medium (SCM307)• 3dGRO(TM) Lung Organoid Maturation Medium (SCM308)Human iPSC Derived AFE Progenitors (SCC301)
Legal Information: 3dGRO is a trademark of Merck KGaA, Darmstadt, Germany