Synonym: BM blue, Sure Blue TMB, TMB Blotting Plus, TMB substrate, TMB
CAS Number: 54827-17-7
Linear Formula: [-C6H2(CH3)2-4-NH2]2
Molecular Weight: 240.34
Beilstein Registry Number: 2808541
EC Number: 259-364-6
MDL Number: MFCD00007748
PubChem Substance ID: 24888954
Application
3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.
Quality Level: PREMIUM
Assay: ≥98.0% (NT)
MP: 166-171 °C; 168-171 °C(lit.)
Storage Temp.: 2-8°C