3,3'-Diaminobenzidine tetrahydrochloride, tablet, 10 mg substrate per tablet

Synonym: 3,3',4,4'-Biphenyltetramine tetrahydrochloride, 3,3',4,4'-Tetraaminobiphenyl tetrahydrochloride, DAB

Linear Formula: (NH₂)₂C₆H₃C₆H₃(NH₂)₂ · 4HCl

Molecular Weight: 360-11

Beilstein Registry Number: 1212988

EC Number: 231-018-9

MDL Number: MFCD00012969

PubChem Substance ID: 24893981

Application
Reagent for spectrophotometric determination of selenium.

Reconstitution
Dissolve 1 tablet in 15 ml of Tris-buffered saline, pH 7.6. Add 12 µl of fresh 30% hydrogen peroxide. DAB and hydrogen peroxide concentration may be adjusted to suit individual applications.

Specificity
Peroxidase substrate suitable for use in immunoblotting and immunohistological staining procedures. This substrate produces an insoluble end product that is brown in color and can be observed visually.