2X SDS-PAGE Sample Loading Buffer is suitable for laboratory assays involved in protein biochemistry. 2X SDS-PAGE Sample Buffer is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. 2X SDS-PAGE Sample Buffer consists of 0.125 M Tris, 4% (w/v) SDS, 20% (v/v) Glycerol and 0.01% (w/v) bromophenol blue. This product is not reduced and does not contain DTT or b-ME. The pH of this solution is 6.8. It was meticulously prepared using ultra pure reagents dissolved in highly polished pharmaceutical grade deionized water.