2,6-dichlorophenolindophenol (DCPIP) is a blue chemical compound used as a redox dye. In Oxidized form DCPIP is blue; whereas in reduced form DCPIP is colorless. The rate of photosynthesis can be measured by the rate at which this dye changes color (is reduced) when exposed to light in a photosynthetic system. This reaction is reversible, as colorless DCPIP can be reoxidized to blue.
2,6-dichlorophenolindophenol sodium salt is a redox dye commonly used as a monitor of the light reactions in photosynthesis because it is an electron acceptor that is blue when oxidized and colorless when reduced. It is part of the Hill reagents family. DCPIP can also be used as an indicator for Vitamin C. If vitamin C, which is a good reducing agent, is present, the blue dye, which turns pink in acid conditions, is reduced to a colorless compound by ascorbic acid.
2,6-dichlorophenolindophenol (DCPIP) is used as a substitute for NADP+. The dye changes color when it is reduced, due to its chemical structure. The rate of photosynthesis light-dependent reaction can be measured with this property of DCPIP, because one of the stages of the light reaction is an electron transport chain that normally ends with the reduction of NADP+. When DCPIP is present, it also gets reduced by the light reaction. The amount of DCPIP reduced can be found by measuring the solution's light transmittance with a spectrophotometer.
Grade: Indicator grade
Suitability: Suitable for vitamin C determination
Key Applications: Indicator for Vitamin C
Application Areas: Cell Biology & Analysis
Product Type: Biochemicals
Biochemical Category: Biochemicals
UV/Visible Absorbance: 604 ± 5 nm λmax (water)
Presentation: Dark Green Powder
Format: Powder
Moisture content: <12%
Solubility: Freely soluble in water, alcohol; soluble in 0.1%(50% ethanol-clear blue solution), (ethanol + 1 N 1mL Na2CO3).
Storage & Handling: Store at Room Temperature (15-30°C). Store Desiccated.