Synonyms: beta-Mercaptoethanol; 2-Hydroxyethylmercaptan; BME; Thioethylene glycol
CAS Number: 60-24-2
Formula: C2H6OS
Formula Weight: 78.13
Linear Formula: HSCH2CH2OH
MDL No.: MFCD00004890
Purity: >=99.0% (GC)
Boiling Point: 157 C(lit.)
Density: 1.114 g/mL at 25 C(lit.)
Refractive Index: n20/D 1.500(lit.); n20/D 1.501
Storage: 2-8C
Beilstein Registry Number: 773648
EC Number: 200-464-6
UNSPSC Code: 12352200
Application: BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.
Application: 2-Mercaptoethanol has been used . in the protein extraction buffer prepared for leaf samples. . as a supplement in cell culture media. . in the procedure of RNA extraction.
Other Notes: Isolation of RNA using guanidinium salts. Inclusion of a reductant enhances denaturation by breaking intramolecular protein disulfide bonds
RTECS: KL5600000
RIDADR: UN 2966 6.1 / PGII
WGK Germany: 3
Flash Point(F): 165.2 F
Flash Point(C): 74 C
R Codes: 23/24/25-38-41-43-48/22-50/53
S Codes: 26-36/37/39-45-60-61
IOD Codes: T,N
Symbol: GHS05, GHS06, GHS08, GHS09
Signal Word: Danger
Hazard Statements: H301 + H331-H310-H315-H317-H318-H373-H410
Precautionary statements: P261-P273-P280-P301 + P310-P302 + P350-P305 + P351 + P338
Target Organs: Heart, Liver