Tris Buffered Saline (TBS) is a solution commonly used in biological and biochemical research. It consists of Tris (tris(hydroxymethyl)aminomethane), which is a buffering agent that maintains the pH of the solution, and Saline (sodium chloride, NaCl), which provides the ionic strength similar to that of physiological fluids.
Uses
Buffering: Maintains a stable pH environment for biological reactions.
Washing: Used to wash membranes in Western blotting.
Dilution: Acts as a diluent for antibodies and other reagents.