G-Biosciences 10B Chemically Competent E. coli is a derivative of the popular DH10B. It is T1 phage resistant and endA deficient for high-quality plasmid preparations. Chemically competent 10B E. coli cells suitable for high efficiency transformation in a wide variety of applications.
Features:
• Clone large plasmids and BACs
• Reduced recombination of cloned DNA (recA1)
• Suitable for blue/white screening by α-complementation of the β-galactosidase gene
• Resistance to phage T1 (fhuA)
• Efficient transformation of unmethylated DNA derived from PCR, cDNA and many other sources (hsdR)
Genotype:
Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14- ?80dlacZΔM15 recA1 relA1 endA1 nupG rpsL (StrR) rph spoT1 Δ(mrr-hsdRMS-mcrBC)
Transformation efficiency: 1 x 109 cfu/µg pUC19 DNA