5 mL, Chemicals, 41115700
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Protein-A Red Separopore®(Agarose)4B
bioWORLDAffinity matrix with immobilized Protein-A by CNBr activation coupling method. For purification and/or fractionation of IgG subclasses. Reliable tool to purify monoclonal and polyclonal IgG from ascies, serum and cell culture supernatants. Supplied as 25% suspension inPBS with sodium…
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Pre-activated medium for immobilization of ligands containing primary amines N, N'-Carbonyl diimidazole (CDI) mediated activation of Separopore® 4B matrix to allow coupling of the primary amino ligands by a stable amide bond formation No charge is introduced into the matrix after the coupling…
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Streptavidin Separopore® (Agarose)
bioWORLDApplications: Affinity matrix with immobilized Streptavidin by CNBr activation coupling method. The base matrix is a rigid, highly cross-linked beaded agarose with high chemical stability. The immobilized streptavidin binds biotin and biotinylated…
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Wheat germ (Triticum vulgaris) agglutinin (WGA) is a 36,000 molecular weight protein consisting of two identical subunits. WGA contains a group of closely related isolectins, with an isoelectric point about pH 9. The receptor sugar for WGA is N-acetylglucosamine. WGA can bind oligosaccharides…
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N-Acetyl-glucosamine-Separopore® 4B-CL is used to purify wheat germ (Triticum vulgaris) lectin, other lectins, and certain other carbohydrate-binding proteins. The purification results in specificity and prolonged biological activity of the protein depending on the applied conditions.…
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BlackPearl®-L (Wheat germ Lectin)
bioWORLDWheat germ lectin (Isolated from Triticum vulgaris) conjugated to BlackPearl® Magnetic Beads. Wheat germ agglutinin is a 36,000 molecular weight protein consisting of two identical subunits. WGA contains a group of closely related isolectins, with an isoelectric point about pH 9. The receptor…
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N-Acetyl-D-galactosamine-Separopore® 4B-CL is an agarose affinity chromatography matrix used to purify carbohydrate-binding proteins such as membrane receptors, and also lectins with affinity to N-Acetyl-galactosamine. N-Acetyl-D-galactosamine-Separopore® 4B-CL has been used to study periodontal…
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L-Phenylalanine Separopore®(Agarose)
bioWORLDApplication: L-Phenylalanine-Separopore® 4B-CL is used for the purification of phenylalanine binding proteins, such as phenylalanine hydroxylase.
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Rapid immobilization of antibodies and other proteins to the matrix Immobilization of ligands through primary amines to purify recombinant proteins Immobilization of Protein A or Protein G for monoclonal antibody purification Pre-activated medium for immobilization of ligands containing a…
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bio-World provides immobilized E. coli proteins on a solid bio-affinity support for the removal of antibodies to E. coliproteins from antisera prepared against recombinant proteins expressed in E. coli. Our immobilized E. coli lysate consists of partially purified proteins from E. coli strains…
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CDI-activated Separopore® Agarose6B
bioWORLDPre-activated medium for immobilization of ligands containing primary amines N,N\'-Carbonyl diimidazole (CDI) mediated activation of Separopore® matrix to allow coupling of the primary amino ligands by a stable amide bond formation No charge is introduced into the matrix after the…
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Gelatin Separopore® 4B has been designed for the purification or removal of fibronectin. Gelatin binds specifically to fibronectin, which is a high molecular weight glycoprotein found on the surfaces of many cell types and present in many extracellular fluids including plasma.
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GTP Separopore®
bioWORLDApplications: GTP-Separopore® is used for purification of GTP-dependent enzymes and proteins that bind nucleotides and related molecules. The GTP-Separopore® 4B-CL resin comprises GTP attached to agarose beads via its γ-phosphate. A long hydrophilic spacer (14-atom) is used to…
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Used for purification of anti-IgG antibodies or to purify Protein A. An affinity chromatography gel matrix consisting of rabbit IgG bound to 4% agarose beads (Separopore® 4B). This matrix is coupled through a stable, leak resistant linkage over a wide pH range. May also be used to immunoprecipitate…
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Anti-Bovine Albumin (BSA)Red Separo
bioWORLDApplication: For the purification of Bovine Albumin (BSA).
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Protein G binds specifically to Fc regions of many mammalian immunoglobulins Protein G conjugates are commonly used as affinity adsorbents to purify immunoglobulins (antibodies) and immunoglobulin subtypes from serum, hybridoma ascites fluids, tissue culture supernatants and other biological…
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Metal ion-chelating Separopore® 4B-CL is designed for the affinity purification of 6X Histidine-tagged proteins. Nickel, Cobalt, Copper and Zinc are used as cations for this type of purification because they provide effective binding and selectivity for a wide range of proteins. Affinity matrix for…
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Red Separopore® 4B-CL
bioWORLDApplications: The immobilized red dye will bind to a wide variety of NADP + - and NAD + -dependent enzymes, including many kinases, dehydrogenases and transferases. Carboxypeptidase G, interferon, plasminogen and plasminogen activator are examples of other proteins that can be purified on…
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Avidin Separopore® (Agarose) 6B
bioWORLDApplications: For the purification of biotin containing proteins.
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Papain-Separopore® 4B can be used as substitute of free papain in many applications. Papain is a cysteine protease that cleaves peptide bonds of basic amino acids, leucine or glycine. It also hydrolyzes esters and amides. This matrix is used to produce Fab fragment of IgG antibodies. It is also…
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Thiophilic Separopore® Agarose 4B-CL
bioWORLDApplications: Thiophilic affinity purification is based on the ability of some proteins, particularly immunoglobulins, to bind to this immobilized ligand. The term "Thiophilic" refers to an affinity for sulfone groups that lie in close proximity to thioether groups. Thiophilic adsorption…
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Affinity matrix for stable carbodiimide-based coupling of ligands containing amino groups Ligands are coupled in a single-step procedure in the presence of a coupling reagent (not provided) which is a water-soluble carbodiimide, such as N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide…
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Calmodulin-Separopore® 4B is used for affinity purification of calmodulin-binding proteins including calmodulin-regulated proteins in eukaryotic cells and recombinant proteins containing calmodulin-binding peptide fusion tag. Calmodulin binds proteins principally through their interactions with…
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EAH-Separopore® 4B is used to couple molecules containing carboxyl groups to the matrix for affinity chromatography. It was prepared by covalent coupling of 1, 6-diamino hexane to Separopore® 4B via epoxy activation. The free terminal amino group allows for coupling of carboxyl-group containing…
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Glucose Separopore® (Agarose) 4B-CL
bioWORLDApplications: β-D-Glucose-Separopore® 4B-CL is an agarose conjugate in saline suspension used in affinity chromatography, protein chromatography and carbohydrate matrices. b-D-Glucose-Separopore® 4B-CL has been used in studies to inform pathogenesis of protozoal infections.…
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IgG (Mouse) Separopore® (Agarose)4B
bioWORLDAn affinity chromatography gel matrix consiting of mouse IgG bound to 4% agarose beads. This matrix is coupled through a stable, leak resistant linkage over a wide pH range. This allows purification of anti-IgG antibodies. May also be used to immunoprecipitate Protein A produced by prokaryotic…
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Glycine Separopore® (Agarose) 4B
bioWORLDApplications: Used for the purification of synaptic membrane proteins and other proteins with affinity for glycine
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L-Aspartic Acid Separopore®(Agarose)
bioWORLDApplications : L-Aspartic Acid-Separopore® 4B-CL is used for the purification of retinol, or other L-Aspartic Acid binding proteins.
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2-Iminobiotin Separopore® Ag. 6B
bioWORLDApplications: 2-Iminobiotin-Separopore® 6B-CL is used for the purification of avidin/streptavidin conjugates. Proteins coupled to (strept)avidin are more readily recovered in native form from 2-Iminobiotin-Separopore® than the biotin resin. It is also used for removal of free avidin from…
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L-Lysine Separopore® (Agarose)4B-CL
bioWORLDApplication: L-Lysine-Separopore® 4B-CL is used for purification of molecules with biospecific or charge dependent affinity for L-lysine. It is also used for the purification of lysine-binding proteins from serum and to purify plasminogen, plasminogen activator, ribosomal RNA, and…
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Brown Separopore® (Agarose) 4B-CL
bioWORLDA dye-ligand affinity matrix consisting of Reactive Brown 10 bound to Cross-linked 4% agarose beads (Separopore® 4B-CL).
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viewIP™ Separopore®
bioWORLDRed-colored agarose matrix contains immobilized Streptavidin for convenient affinity capture (pull-down) of biotinylated target molecules, such as proteins, peptides, antibodies, nucleic acids, lectins, receptors and ligands. viewIP TM Red Affinity Gels greatly reduce the risk of pellet loss.…