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NHS-activated Separopore® (Agarose) 4B-CL
- Rapid immobilization of antibodies and other proteins to the matrix
- Immobilization of ligands through primary amines to purify recombinant proteins
- Immobilization of Protein A or Protein G for monoclonal antibody purification
- Pre-activated medium for immobilization of ligands containing a primary amino group
- N-hydroxyl succinimide (NHS) ester forms a stable amide (peptide) bond with the primary amines of ligands
- The stability of an amide bond at high pH (up to pH 13) allows for a very stringent wash protocol during affinity purification using NHS-activated ligand coupling
- Coupling can be carried out in neutral pH which makes it ideal for immobilization of antibodies, antigens, and other proteins sensitive to pH extremes
- Coupling reaction can also be carried out in organic solvents
- Provides a 10 atom spacer arm upon ligand coupling
- Coupling completed after 2 – 4 h at room temperature
- NHS-Activated Separopore® provides a valuable tool for affinity purification of antibodies, antigens and other biomolecules
| Group to be coupled: | Primary -NH2 |
| Matrix: | Separopore® 4B-CL (highly crosslinked agarose beads, 4%) |
| Particle size range: | 52 – 165 µm |
| Molecular weight range: | 6 x 104 – 2 x 107 |
| Chemical linkage of ligand: | Amide bond |
| Spacer arm: | 6-aminocaproic acid, 10 atoms |
| Substitution: | >18 µmol NHS / ml drained gel |
| pH stability: | 3 – 13 (ligand dependent) |
| Coupling conditions: | pH 6 – 9; Temp: 4 – 25 °C |
| Storage: | 2 – 8 °C |
| Flow rate specifications: | 70 – 140 cm / h |
| Supplied as a pre-swelled gel suspension in 100% isopropanol to protect the active groups |
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Product Detail
- Description
- NHS-Activated Separopore® 4B-CL (With Spacer Arm), 5 mL
- Packaging Size
- 5 mL
- UN Number
- 41115700
| list price/quantity | total | | $0.00 |
- Description
- NHS-Activated Separopore® 4B-CL (With Spacer Arm), 10 mL
- Packaging Size
- 10 mL
- UN Number
- 41115700
| list price/quantity | total | | $0.00 |
- Description
- NHS-Activated Separopore® 4B-CL (With Spacer Arm), 25 mL
- Packaging Size
- 25 mL
- UN Number
- 41115700
| list price/quantity | total | | $0.00 |
- Description
- NHS-Activated Separopore® 4B-CL (With Spacer Arm), 50 mL
- Packaging Size
- 50 mL
- UN Number
- 41115700
| list price/quantity | total | | $0.00 |
Product is restricted and can only be purchased by customers with a web profile linked to a Thomas Business Account -
Click here to login or
create your web profile. If you do not have a Thomas Business Account, please
Contact Us and someone will respond with details on how to apply for a business account with us.