Separopore Bioaffinity Matrices

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Separopore Bioaffinity Matrices

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  • CNBr-activated Separopore® Agarose 4B

    Cyanogen bromide-activated Separopore® 4B matrix is used for preparation of resins for affinity chromatography. Pre-activated medium is suitable for immobilization of ligands containing primary amines. Cyanogen bromide (CNBr) in base reacts with hydroxyl groups on Separopore® 4B to form cyanate…

  • Heparin Separopore® (Agarose) 4B

    Heparin Separopore® 4B is an affinity matrix widely used in affinity purification of various heparin-binding proteins or ligands, such as antithrombin III, lipoprotein, DNA binding proteins (transcription factors, virus coat proteins etc.) and steroid receptors. It can also be used as a high…

  • Streptavidin Separopore® (Agarose)

    bioWORLD

    Applications: Affinity matrix with immobilized Streptavidin by CNBr activation coupling method. The base matrix is a rigid, highly cross-linked beaded agarose with high chemical stability. The immobilized streptavidin binds biotin and biotinylated…

  • Anti-Bovine Albumin BSA Separopore®

    bioWORLD

    Application: Anti-BSA-Separopore® 4B is used for immunoprecipitation and immunoaffinity purification of bovine serum albumin (BSA) from solutions (and co-immunoprecipitation of biomolecules that bind BSA). It is also used to remove BSA from solutions.

  • Papain Separopore® (Agarose) 4B

    Papain-Separopore® 4B can be used as substitute of free papain in many applications. Papain is a cysteine protease that cleaves peptide bonds of basic amino acids, leucine or glycine. It also hydrolyzes esters and amides. This matrix is used to produce Fab fragment of IgG antibodies. It is also…

  • Separopore® (Agarose) 2B

    bioWORLD

    Applications: Separopore® 2B is a high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands. It is ideal for for both analytical and preparative protein purifications. Separopore® 2B is a proven base matrix for coupling affinity ligands. Separopore® 2B provides a good…

  • Cu-Chelated Separopore® Agarose

    bioWORLD

    Cu-Chelated Separopore® is an imminodiacetate (IDA) immobilized metal affinity chromatography (IMAC) matrix that seperates proteins via their differences in affinity to Cu metal ions. Properties Affinity matrix for proteins. Imminodiacetate immobilized matrix charged with Cu metal ions.v…

  • Aldehyde Separopore® (Agarose) 4B-CL

    bioWORLD

    Applications: Aldehyde Separopore® 4B has a robust reductive amino alkylation coupling chemistry to immobilize proteins and other ligands through primary amines (–NH2) to the resin. It can be used for a variety of affinity purification methods involving batch or column chromatography.…

  • Calmodulin Separopore® (Agarose)

    Calmodulin-Separopore® 4B is used for affinity purification of calmodulin-binding proteins including calmodulin-regulated proteins in eukaryotic cells and recombinant proteins containing calmodulin-binding peptide fusion tag. Calmodulin binds proteins principally through their interactions with…

  • Dextran Separopore® (Agarose)4B-CL

    Dextran is coupled to a high strength Separopore® 4B-CL matrix. Coupling dextran to this material expands the surface area of the Separopore® beads allowing for further binding of macromolecules. Dextran-Separopore® 4B-CL is used in affinity chromatography as a protein binding site. It has been…

  • Tosyl-activated Separopore® Agarose

    bioWORLD

    Applications: Tosyl-activated Separopore® 4B-CL is used to couple primary amines, thiols, imidazole or tyrosine hydroxyl groups to the matrix. Tosyl-activated Separopore® 4B-CL couples more rapidly and efficiently to sulfhydryl groups than to primary amines. Coupling of antibodies through…

  • Glutathione Separopore® (Agarose) 4B

    bioWORLD

    Applications: Reduced glutathione is covalently linked Separopore® 4B for use in affinity purification of glutathione-S-transferase (GST) and GST-fused proteins. This product provides a one step purification method and permits rapid, mild and highly selective purifications of proteins…

  • Lactose Separopore® (Agarose)6B-CL

    a-Lactose-Separopore® 4B-CL is used in protein chromatography, affinity chromatography and carbohydrate matrices. a-Lactose-Separopore® 4B-CL has been used to study microneme proteins and host-parasite cell surface receptors. Lactose-Separopore® 4B-CL may be used to isolate lactose binding…

  • N-Acetyl-glucosamine (GlcNAc) Separopore® (Agarose) 4B-CL

    N-Acetyl-glucosamine-Separopore® 4B-CL is used to purify wheat germ (Triticum vulgaris) lectin, other lectins, and certain other carbohydrate-binding proteins. The purification results in specificity and prolonged biological activity of the protein depending on the applied conditions.…

  • NADP-Separopore® (Agarose) 4B-CL (Lyophilized)

    NADP covalently bound to Separopore® has a high affinity for NADP-dependent enzymes and dehydrogenases. Acetyl-CoA binds to NADP-Separopore® 4B-CL twelve times higher than NAD-Separopore® 4B-CL. NADP-Separopore® 4B-CL has also been used to purify extracellular nucleases.

  • DEAE Separopore® (Agarose) 6B-CL

    DEAE-Separopore® 6B-CL is a weak anion exchanger with excellent flow capacities and a high capacity for binding and resolution of proteins of all pI values. bio-World's DEAE-Separopore® 6B-CL was prepared by covalent attachment of diethylaminoethyl (DEAE) to Separopore® 6B-CL. DEAE remains charged…

  • Protein G-Separopore® (Agarose) 4B-CL

    Protein G binds specifically to Fc regions of many mammalian immunoglobulins Protein G conjugates are commonly used as affinity adsorbents to purify immunoglobulins (antibodies) and immunoglobulin subtypes from serum, hybridoma ascites fluids, tissue culture supernatants and other biological…

  • Protein A-Separopore® (Agarose) 4B-CL (Lyophilized)

    Protein A binds specifically to Fc regions of many mammalian immunoglobulins. Protein A- conjugates are commonly used as affinity adsorbents to purify immunoglobulins (antibodies) and immunoglobulin subtypes from serum, hybridoma ascites fluids, tissue culture supernatants and other biological…

  • KLH Separopore® (Agarose) 4B

    Affinity matrix with immobilized KLH used for the purification of antibodies generated against peptide-KLH conjugated antigens byremoving antibodies against the KLH portion of the antigen. Anti-KLH antibodies can be removed by solid phase immuno-affinity column chromatography over the KLH-…

  • IDA Chelating Separopore® (Agarose) 4B-CL

    Metal ion-chelating Separopore® 4B-CL is designed for the affinity purification of 6X Histidine-tagged proteins. Nickel, Cobalt, Copper and Zinc are used as cations for this type of purification because they provide effective binding and selectivity for a wide range of proteins. Affinity matrix for…

  • Separopore® (Agarose) 6B-CL

    bioWORLD

    Separopore® 6B-CL is crosslinked high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands. It is ideal for both analytical and preparative protein purifications. Separopore® 6B-CL is a proven base matrix for coupling affinity ligands. Separopore® 6B-CL…

  • Guar gum Separopore® (Agarose)4B-CL

    bioWORLD

    Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

  • Separopore® (Agarose) 2B-CL

    bioWORLD

    Applications: Separopore® 2B-CL is a cross-linked bioagarose medium for gel filtration and coupling of affinity ligands. Hydrophilic backbone of Separopore® 2B-CL results in low non-specific interactions and high protein recoveries. Separopore® 2B-CL provides an excellent separation of proteins…

  • Separopore® (Agarose) 6B-MB

    bioWORLD

    Separopore® 6B (macrobeads) is a larger sized agarose macrobead matrix for gel filtration and coupling of affinity ligands. It is ideal for both analytical and preparative protein purifications. Larger volumes available at discounted prices (enquire).

  • Protein-A Blue Separopore® Ag 4B-MB

    bioWORLD

    Applications: Protein A Blue-Separopore® 4B-MB functions same as conventional Protein A affinity beads, but offers enhanced visibility due to blue colored Separopore® macrobeads. The blue colored beads help provide visual monitoring to avoid the accidental loss of beads during pull-down in…

  • Separopore® (Agarose) 6B

    bioWORLD

    Applications: Separopore® 6B is a high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands. It is ideal for both analytical and preparative protein purifications. Separopore® 6B is a proven base matrix for coupling affinity ligands. Separopore® 6B…

  • IgG (Rabbit) Separopore® (Agarose) 4B

    Used for purification of anti-IgG antibodies or to purify Protein A. An affinity chromatography gel matrix consisting of rabbit IgG bound to 4% agarose beads (Separopore® 4B). This matrix is coupled through a stable, leak resistant linkage over a wide pH range. May also be used to immunoprecipitate…

  • Co-Chelated Separopore®

    bioWORLD

    Co-Chelated Separopore® is an imminodiacetate (IDA) immobilized metal affinity chromatography (IMAC) matrix that seperates proteins via their differences in affinity to nickel metal ions. Properties Affinity matrix for proteins. Imminodiacetate immobilized matrix charged with Co metal…

  • 2-Iminobiotin Separopore® Ag. 6B

    bioWORLD

    Applications: 2-Iminobiotin-Separopore® 6B-CL is used for the purification of avidin/streptavidin conjugates. Proteins coupled to (strept)avidin are more readily recovered in native form from 2-Iminobiotin-Separopore® than the biotin resin. It is also used for removal of free avidin from…

  • Ubiquitin Separopore® (Agarose)

    bioWORLD

    Applications: Ubiquitin-Separopore® 4B is useful for binding the ubiquitin activation enzyme (E1), ubiquitin carrier enzymes (E2s), ubiquitin ligases (E3s) and ubiquitin-terminal hydrolyases. Ubiquitin-Separopore® 4B is used in affinity chromatography to study enzymes involved in…

  • Gelatin Separopore® (Agarose) 4B

    Gelatin Separopore® 4B has been designed for the purification or removal of fibronectin. Gelatin binds specifically to fibronectin, which is a high molecular weight glycoprotein found on the surfaces of many cell types and present in many extracellular fluids including plasma.

  • E. coli Protein-Separopore® (Agarose) 4B

    bio-World provides immobilized E. coli proteins on a solid bio-affinity support for the removal of antibodies to E. coliproteins from antisera prepared against recombinant proteins expressed in E. coli. Our immobilized E. coli lysate consists of partially purified proteins from E. coli strains…

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