Separopore Bioaffinity Matrices

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Separopore Bioaffinity Matrices

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  • Heparin Separopore® (Agarose) 4B

    Heparin Separopore® 4B is an affinity matrix widely used in affinity purification of various heparin-binding proteins or ligands, such as antithrombin III, lipoprotein, DNA binding proteins (transcription factors, virus coat proteins etc.) and steroid receptors. It can also be used as a high…

  • Fucose Separopore® (Agarose) 4B-CL

    Fucose-Separopore® 4B-CL used as affinity matrix for the purification of fucose-binding glycoproteins. Fucose is linked to Separopore® 4B-CL via a spacer arm to ensure efficient binding of this ligand to the protein.

  • Separopore® (Agarose) 2B


    Applications: Separopore® 2B is a high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands. It is ideal for for both analytical and preparative protein purifications. Separopore® 2B is a proven base matrix for coupling affinity ligands. Separopore® 2B provides a good…

  • CNBr-activated Separopore® Agarose 4B

    Cyanogen bromide-activated Separopore® 4B matrix is used for preparation of resins for affinity chromatography. Pre-activated medium is suitable for immobilization of ligands containing primary amines. Cyanogen bromide (CNBr) in base reacts with hydroxyl groups on Separopore® 4B to form cyanate…

  • 2-Iminobiotin Separopore® Ag. 6B


    Applications: 2-Iminobiotin-Separopore® 6B-CL is used for the purification of avidin/streptavidin conjugates. Proteins coupled to (strept)avidin are more readily recovered in native form from 2-Iminobiotin-Separopore® than the biotin resin. It is also used for removal of free avidin from…

  • Glutathione Separopore® Microbeads


    Applications: Reduced glutathione is covalently linked Separopore® 4B Microbeads for use in affinity purification of glutathione-S-transferase (GST) and GST-fused proteins. This product provides a one step purification method and permits rapid, mild and highly selective purifications of proteins…

  • Aldehyde Separopore® (Agarose) 4B-CL


    Applications: Aldehyde Separopore® 4B has a robust reductive amino alkylation coupling chemistry to immobilize proteins and other ligands through primary amines (–NH2) to the resin. It can be used for a variety of affinity purification methods involving batch or column chromatography.…

  • Streptavidin Separopore® (Agarose)


    Applications: Affinity matrix with immobilized Streptavidin by CNBr activation coupling method. The base matrix is a rigid, highly cross-linked beaded agarose with high chemical stability. The immobilized streptavidin binds biotin and biotinylated…

  • Tosyl-activated Separopore® Agarose


    Applications: Tosyl-activated Separopore® 4B-CL is used to couple primary amines, thiols, imidazole or tyrosine hydroxyl groups to the matrix. Tosyl-activated Separopore® 4B-CL couples more rapidly and efficiently to sulfhydryl groups than to primary amines. Coupling of antibodies through…

  • Papain Separopore® (Agarose) 4B

    Papain-Separopore® 4B can be used as substitute of free papain in many applications. Papain is a cysteine protease that cleaves peptide bonds of basic amino acids, leucine or glycine. It also hydrolyzes esters and amides. This matrix is used to produce Fab fragment of IgG antibodies. It is also…

  • IDA Chelating Separopore® (Agarose) 4B-CL

    Metal ion-chelating Separopore® 4B-CL is designed for the affinity purification of 6X Histidine-tagged proteins. Nickel, Cobalt, Copper and Zinc are used as cations for this type of purification because they provide effective binding and selectivity for a wide range of proteins. Affinity matrix for…

  • IgG-Separopore® (Agarose)-Fusion Kit


    A complete kit to bind Protein A fusion proteins expressed in E. coli or yeast. Kit consists of affinity chromatography gel matrix, IgG-Separopore® 4B, including all the components/buffers that allow for quick, convenient purification of anti-IgG antibodies. This kit may also be used to…

  • NAD-Separopore® (Agarose) 4B-CL (Lyophilized)

    NAD covalently bound to Separopore® has a high affinity for NAD-dependent enzymes and dehydrogenases. NAD-Separopore® 4B-CL has been used to purify Vibrio cholerae toxin, diptheria toxin and other NAD binding proteins.

  • Separopore® (Agarose) 6B


    Applications: Separopore® 6B is a high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands. It is ideal for both analytical and preparative protein purifications. Separopore® 6B is a proven base matrix for coupling affinity ligands. Separopore® 6B…

  • N-Acetyl-glucosamine (GlcNAc) Separopore® (Agarose) 4B-CL

    N-Acetyl-glucosamine-Separopore® 4B-CL is used to purify wheat germ (Triticum vulgaris) lectin, other lectins, and certain other carbohydrate-binding proteins. The purification results in specificity and prolonged biological activity of the protein depending on the applied conditions.…

  • Galactose Separopore® Agarose 4B-CL

    Galactose coupled Separopore® is a high strength bioagarose matrix. Galactose-Separopore® 4B-CL is used in the purification of agglutinins, toxins, lectins, galactosidases and other galactose-binding molecules, N-acetylgalactosamine-binding proteins or carbohydrate-binding proteins. This…

  • Protein G-Separopore® (Agarose) 4B-CL

    Protein G binds specifically to Fc regions of many mammalian immunoglobulins Protein G conjugates are commonly used as affinity adsorbents to purify immunoglobulins (antibodies) and immunoglobulin subtypes from serum, hybridoma ascites fluids, tissue culture supernatants and other biological…

  • KLH Separopore® (Agarose) 4B

    Affinity matrix with immobilized KLH used for the purification of antibodies generated against peptide-KLH conjugated antigens byremoving antibodies against the KLH portion of the antigen. Anti-KLH antibodies can be removed by solid phase immuno-affinity column chromatography over the KLH-…

  • Co-Chelated Separopore®


    Co-Chelated Separopore® is an imminodiacetate (IDA) immobilized metal affinity chromatography (IMAC) matrix that seperates proteins via their differences in affinity to nickel metal ions. Properties Affinity matrix for proteins. Imminodiacetate immobilized matrix charged with Co metal…

  • Gelatin Separopore® (Agarose) 4B

    Gelatin Separopore® 4B has been designed for the purification or removal of fibronectin. Gelatin binds specifically to fibronectin, which is a high molecular weight glycoprotein found on the surfaces of many cell types and present in many extracellular fluids including plasma.

  • EAH Separopore® (Agarose) 4B

    EAH-Separopore® 4B is used to couple molecules containing carboxyl groups to the matrix for affinity chromatography. It was prepared by covalent coupling of 1, 6-diamino hexane to Separopore® 4B via epoxy activation. The free terminal amino group allows for coupling of carboxyl-group containing…

  • viewIP™ Protein-A Red Separopore® 4B


    Applications: The red-colored agarose bead affinity matrix has immobilized Recombinant Protein A. Convenient for quantitative capturing of antigen/antibody complexes in immunoprecipitation, it greatly reducing the risk of pellet loss. Comparable performance to non-colored affinity gels, but allows…

  • Epoxy-activated Separopore® (Agarose) 4B-CL

    Used in coupling of hydroxyl, amino or thiol groups to Separopore® 4B via a 12-carbon hydrophilic spacer arm Pre-activated medium for immobilization of ligands containing amino (primary or secondary), hydroxyl or thiol group The matrix provides a 10-atom spacer arm convenient for coupling of…

  • ECH Separopore® (Agarose) 4B

    Affinity matrix for stable carbodiimide-based coupling of ligands containing amino groups Ligands are coupled in a single-step procedure in the presence of a coupling reagent (not provided) which is a water-soluble carbodiimide, such as N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide…

  • Separopore® (Agarose) 4B-CL


    Separopore® 4B-CL is crosslinked high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands suitable for analytical and preparative protein purifications. Separopore® 4B-CL is a proven base matrix for coupling affinity ligands. Separopore® 4B-CL provides…

  • Guar gum Separopore® (Agarose)4B-CL


    Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

  • Separopore® (Agarose) 2B-CL


    Applications: Separopore® 2B-CL is a cross-linked bioagarose medium for gel filtration and coupling of affinity ligands. Hydrophilic backbone of Separopore® 2B-CL results in low non-specific interactions and high protein recoveries. Separopore® 2B-CL provides an excellent separation of proteins…

  • Separopore® (Agarose) 6B-MB


    Separopore® 6B (macrobeads) is a larger sized agarose macrobead matrix for gel filtration and coupling of affinity ligands. It is ideal for both analytical and preparative protein purifications. Larger volumes available at discounted prices (enquire).

  • Separopore® (Agarose) 4B


    Separopore® 4B is a high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands. It is ideal for both analytical and preparative protein purifications. Separopore® 4B is a proven base matrix for coupling affinity ligands. Separopore® 4B provides good…

  • Thiophilic Separopore® Agarose 4B-CL


    Applications: Thiophilic affinity purification is based on the ability of some proteins, particularly immunoglobulins, to bind to this immobilized ligand. The term "Thiophilic" refers to an affinity for sulfone groups that lie in close proximity to thioether groups. Thiophilic adsorption…

  • Dextran Separopore® (Agarose)4B-CL

    Dextran is coupled to a high strength Separopore® 4B-CL matrix. Coupling dextran to this material expands the surface area of the Separopore® beads allowing for further binding of macromolecules. Dextran-Separopore® 4B-CL is used in affinity chromatography as a protein binding site. It has been…

  • Calmodulin Separopore® (Agarose)

    Calmodulin-Separopore® 4B is used for affinity purification of calmodulin-binding proteins including calmodulin-regulated proteins in eukaryotic cells and recombinant proteins containing calmodulin-binding peptide fusion tag. Calmodulin binds proteins principally through their interactions with…

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