Separopore Bioaffinity Matrices

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Separopore Bioaffinity Matrices

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  • Fucose Separopore® (Agarose) 4B-CL

    Fucose-Separopore® 4B-CL used as affinity matrix for the purification of fucose-binding glycoproteins. Fucose is linked to Separopore® 4B-CL via a spacer arm to ensure efficient binding of this ligand to the protein.

  • Separopore® (Agarose) 4B-MB

    bioWORLD

    Separopore® 4B (macrobeads) is a larger sized agarose bead matrix for gel filtration and coupling of affinity ligands. It is ideal for both analytical and preparative protein purifications. Larger volumes available at discounted prices (enquire).

  • Gelatin Separopore® (Agarose) 4B

    Gelatin Separopore® 4B has been designed for the purification or removal of fibronectin. Gelatin binds specifically to fibronectin, which is a high molecular weight glycoprotein found on the surfaces of many cell types and present in many extracellular fluids including plasma.

  • Separopore® (Agarose) 4B-CL

    bioWORLD

    Separopore® 4B-CL is crosslinked high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands suitable for analytical and preparative protein purifications. Separopore® 4B-CL is a proven base matrix for coupling affinity ligands. Separopore® 4B-CL provides…

  • Anti-GFP Tag (GF28R), Monoclonal Ab

    bioWORLD

    Anti-GFP Tag (GF28R) mAb recognizes both the native and denatured forms of GFP (Green Fluorescent Protein) and its many popular variants: EGFP, YFP, EYFP, and CFP. Description: Anti-GFP (Green Fluorescent Protein) Tag (GF28R) Monoclonal Antibody Clonality: GF28R Host Species: Mouse …

  • Heparin Separopore® (Agarose) 4B

    Heparin Separopore® 4B is an affinity matrix widely used in affinity purification of various heparin-binding proteins or ligands, such as antithrombin III, lipoprotein, DNA binding proteins (transcription factors, virus coat proteins etc.) and steroid receptors. It can also be used as a high…

  • Blue-Separopore® (Agarose) 6B-CL

    Cibacron Blue 3GA-Separopore® 6B-CL is an agarose conjugate in saline suspension used to searate and purify a variety of proteins. The ligand Cibacron Blue F3G-A is covalently coupled to Separopore® via chlorotriazine ring. The structure of the blue dye makes it a very versatile tool for separating…

  • Separopore® (Agarose) 4B

    bioWORLD

    Separopore® 4B is a high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands. It is ideal for both analytical and preparative protein purifications. Separopore® 4B is a proven base matrix for coupling affinity ligands. Separopore® 4B provides good…

  • N-Acetyl-glucosamine (GlcNAc) Separopore® (Agarose) 4B-CL

    N-Acetyl-glucosamine-Separopore® 4B-CL is used to purify wheat germ (Triticum vulgaris) lectin, other lectins, and certain other carbohydrate-binding proteins. The purification results in specificity and prolonged biological activity of the protein depending on the applied conditions.…

  • Separopore® (Agarose) 2B

    bioWORLD

    Applications: Separopore® 2B is a high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands. It is ideal for for both analytical and preparative protein purifications. Separopore® 2B is a proven base matrix for coupling affinity ligands. Separopore® 2B provides a good…

  • CNBr-activated Separopore® Agarose 4B

    Cyanogen bromide-activated Separopore® 4B matrix is used for preparation of resins for affinity chromatography. Pre-activated medium is suitable for immobilization of ligands containing primary amines. Cyanogen bromide (CNBr) in base reacts with hydroxyl groups on Separopore® 4B to form cyanate…

  • NHS-activated Separopore® (Agarose) 4B-CL

    Rapid immobilization of antibodies and other proteins to the matrix Immobilization of ligands through primary amines to purify recombinant proteins Immobilization of Protein A or Protein G for monoclonal antibody purification Pre-activated medium for immobilization of ligands containing a…

  • Papain Separopore® (Agarose) 4B

    Papain-Separopore® 4B can be used as substitute of free papain in many applications. Papain is a cysteine protease that cleaves peptide bonds of basic amino acids, leucine or glycine. It also hydrolyzes esters and amides. This matrix is used to produce Fab fragment of IgG antibodies. It is also…

  • Co-Chelated Separopore®

    bioWORLD

    Co-Chelated Separopore® is an imminodiacetate (IDA) immobilized metal affinity chromatography (IMAC) matrix that seperates proteins via their differences in affinity to nickel metal ions. Properties Affinity matrix for proteins. Imminodiacetate immobilized matrix charged with Co metal…

  • Zn-Chelated Separopore® Agarose

    bioWORLD

    Zn-Chelated Separopore® is an imminodiacetate (IDA) immobilized metal affinity chromatography (IMAC) matrix that seperates proteins via their differences in affinity to Zn metal ions. Properties Affinity matrix for proteins. Imminodiacetate immobilized matrix charged with Zu metal…

  • Separopore® (Agarose) 6B

    bioWORLD

    Applications: Separopore® 6B is a high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands. It is ideal for both analytical and preparative protein purifications. Separopore® 6B is a proven base matrix for coupling affinity ligands. Separopore® 6B…

  • DEAE Separopore® (Agarose) 6B-CL

    DEAE-Separopore® 6B-CL is a weak anion exchanger with excellent flow capacities and a high capacity for binding and resolution of proteins of all pI values. bio-World's DEAE-Separopore® 6B-CL was prepared by covalent attachment of diethylaminoethyl (DEAE) to Separopore® 6B-CL. DEAE remains charged…

  • Aldehyde Separopore® (Agarose) 4B-CL

    bioWORLD

    Applications: Aldehyde Separopore® 4B has a robust reductive amino alkylation coupling chemistry to immobilize proteins and other ligands through primary amines (–NH2) to the resin. It can be used for a variety of affinity purification methods involving batch or column chromatography.…

  • Galactose Separopore® Agarose 4B-CL

    Galactose coupled Separopore® is a high strength bioagarose matrix. Galactose-Separopore® 4B-CL is used in the purification of agglutinins, toxins, lectins, galactosidases and other galactose-binding molecules, N-acetylgalactosamine-binding proteins or carbohydrate-binding proteins. This…

  • Streptavidin Separopore® (Agarose)

    bioWORLD

    Applications: Affinity matrix with immobilized Streptavidin by CNBr activation coupling method. The base matrix is a rigid, highly cross-linked beaded agarose with high chemical stability. The immobilized streptavidin binds biotin and biotinylated…

  • Glutathione Separopore® (Agarose) 4B

    bioWORLD

    Applications: Reduced glutathione is covalently linked Separopore® 4B for use in affinity purification of glutathione-S-transferase (GST) and GST-fused proteins. This product provides a one step purification method and permits rapid, mild and highly selective purifications of proteins…

  • Protein G-Separopore® (Agarose) 4B-CL

    Protein G binds specifically to Fc regions of many mammalian immunoglobulins Protein G conjugates are commonly used as affinity adsorbents to purify immunoglobulins (antibodies) and immunoglobulin subtypes from serum, hybridoma ascites fluids, tissue culture supernatants and other biological…

  • Protein A-Separopore® (Agarose) 4B-CL (Lyophilized)

    Protein A binds specifically to Fc regions of many mammalian immunoglobulins. Protein A- conjugates are commonly used as affinity adsorbents to purify immunoglobulins (antibodies) and immunoglobulin subtypes from serum, hybridoma ascites fluids, tissue culture supernatants and other biological…

  • EAH Separopore® (Agarose) 4B

    EAH-Separopore® 4B is used to couple molecules containing carboxyl groups to the matrix for affinity chromatography. It was prepared by covalent coupling of 1, 6-diamino hexane to Separopore® 4B via epoxy activation. The free terminal amino group allows for coupling of carboxyl-group containing…

  • ECH Separopore® (Agarose) 4B

    Affinity matrix for stable carbodiimide-based coupling of ligands containing amino groups Ligands are coupled in a single-step procedure in the presence of a coupling reagent (not provided) which is a water-soluble carbodiimide, such as N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide…

  • CDI-Activated Separopore® Agarose 4B

    Pre-activated medium for immobilization of ligands containing primary amines N, N'-Carbonyl diimidazole (CDI) mediated activation of Separopore® 4B matrix to allow coupling of the primary amino ligands by a stable amide bond formation No charge is introduced into the matrix after the coupling…

  • Protein-A Blue Separopore® Ag 4B-MB

    bioWORLD

    Applications: Protein A Blue-Separopore® 4B-MB functions same as conventional Protein A affinity beads, but offers enhanced visibility due to blue colored Separopore® macrobeads. The blue colored beads help provide visual monitoring to avoid the accidental loss of beads during pull-down in…

  • Dextran Separopore® (Agarose)4B-CL

    Dextran is coupled to a high strength Separopore® 4B-CL matrix. Coupling dextran to this material expands the surface area of the Separopore® beads allowing for further binding of macromolecules. Dextran-Separopore® 4B-CL is used in affinity chromatography as a protein binding site. It has been…

  • IgG (Rabbit) Separopore® (Agarose) 4B

    Used for purification of anti-IgG antibodies or to purify Protein A. An affinity chromatography gel matrix consisting of rabbit IgG bound to 4% agarose beads (Separopore® 4B). This matrix is coupled through a stable, leak resistant linkage over a wide pH range. May also be used to immunoprecipitate…

  • 2-Iminobiotin Separopore® Ag. 6B

    bioWORLD

    Applications: 2-Iminobiotin-Separopore® 6B-CL is used for the purification of avidin/streptavidin conjugates. Proteins coupled to (strept)avidin are more readily recovered in native form from 2-Iminobiotin-Separopore® than the biotin resin. It is also used for removal of free avidin from…

  • Ubiquitin Separopore® (Agarose)

    bioWORLD

    Applications: Ubiquitin-Separopore® 4B is useful for binding the ubiquitin activation enzyme (E1), ubiquitin carrier enzymes (E2s), ubiquitin ligases (E3s) and ubiquitin-terminal hydrolyases. Ubiquitin-Separopore® 4B is used in affinity chromatography to study enzymes involved in…

  • NAD-Separopore® (Agarose) 4B-CL (Lyophilized)

    NAD covalently bound to Separopore® has a high affinity for NAD-dependent enzymes and dehydrogenases. NAD-Separopore® 4B-CL has been used to purify Vibrio cholerae toxin, diptheria toxin and other NAD binding proteins.

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