Separopore Bioaffinity Matrices

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Separopore Bioaffinity Matrices

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  • Separopore® (Agarose) 4B-CL

    bioWORLD

    Separopore® 4B-CL is crosslinked high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands suitable for analytical and preparative protein purifications. Separopore® 4B-CL is a proven base matrix for coupling affinity ligands. Separopore® 4B-CL provides…

  • 2-Iminobiotin Separopore® Ag. 6B

    bioWORLD

    Applications: 2-Iminobiotin-Separopore® 6B-CL is used for the purification of avidin/streptavidin conjugates. Proteins coupled to (strept)avidin are more readily recovered in native form from 2-Iminobiotin-Separopore® than the biotin resin. It is also used for removal of free avidin from…

  • Glutathione Separopore® (Agarose) 4B

    bioWORLD

    Applications: Reduced glutathione is covalently linked Separopore® 4B for use in affinity purification of glutathione-S-transferase (GST) and GST-fused proteins. This product provides a one step purification method and permits rapid, mild and highly selective purifications of proteins…

  • Separopore® (Agarose) 2B

    bioWORLD

    Applications: Separopore® 2B is a high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands. It is ideal for for both analytical and preparative protein purifications. Separopore® 2B is a proven base matrix for coupling affinity ligands. Separopore® 2B provides a good…

  • viewIP™ Protein-A Red Separopore® 4B

    bioWORLD

    Applications: The red-colored agarose bead affinity matrix has immobilized Recombinant Protein A. Convenient for quantitative capturing of antigen/antibody complexes in immunoprecipitation, it greatly reducing the risk of pellet loss. Comparable performance to non-colored affinity gels, but allows…

  • Protein G-Separopore® (Agarose) 4B-CL

    Protein G binds specifically to Fc regions of many mammalian immunoglobulins Protein G conjugates are commonly used as affinity adsorbents to purify immunoglobulins (antibodies) and immunoglobulin subtypes from serum, hybridoma ascites fluids, tissue culture supernatants and other biological…

  • Streptavidin Separopore® (Agarose)

    bioWORLD

    Applications: Affinity matrix with immobilized Streptavidin by CNBr activation coupling method. The base matrix is a rigid, highly cross-linked beaded agarose with high chemical stability. The immobilized streptavidin binds biotin and biotinylated…

  • NHS-activated Separopore® (Agarose) 4B-CL

    Rapid immobilization of antibodies and other proteins to the matrix Immobilization of ligands through primary amines to purify recombinant proteins Immobilization of Protein A or Protein G for monoclonal antibody purification Pre-activated medium for immobilization of ligands containing a…

  • Epoxy-activated Separopore® (Agarose) 4B-CL

    Used in coupling of hydroxyl, amino or thiol groups to Separopore® 4B via a 12-carbon hydrophilic spacer arm Pre-activated medium for immobilization of ligands containing amino (primary or secondary), hydroxyl or thiol group The matrix provides a 10-atom spacer arm convenient for coupling of…

  • Separopore® (Agarose) 2B-CL

    bioWORLD

    Applications: Separopore® 2B-CL is a cross-linked bioagarose medium for gel filtration and coupling of affinity ligands. Hydrophilic backbone of Separopore® 2B-CL results in low non-specific interactions and high protein recoveries. Separopore® 2B-CL provides an excellent separation of proteins…

  • Separopore® (Agarose) 6B-MB

    bioWORLD

    Separopore® 6B (macrobeads) is a larger sized agarose macrobead matrix for gel filtration and coupling of affinity ligands. It is ideal for both analytical and preparative protein purifications. Larger volumes available at discounted prices (enquire).

  • Galactose Separopore® Agarose 4B-CL

    Galactose coupled Separopore® is a high strength bioagarose matrix. Galactose-Separopore® 4B-CL is used in the purification of agglutinins, toxins, lectins, galactosidases and other galactose-binding molecules, N-acetylgalactosamine-binding proteins or carbohydrate-binding proteins. This…

  • Gelatin Separopore® (Agarose) 4B

    Gelatin Separopore® 4B has been designed for the purification or removal of fibronectin. Gelatin binds specifically to fibronectin, which is a high molecular weight glycoprotein found on the surfaces of many cell types and present in many extracellular fluids including plasma.

  • Separopore® (Agarose) 4B

    bioWORLD

    Separopore® 4B is a high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands. It is ideal for both analytical and preparative protein purifications. Separopore® 4B is a proven base matrix for coupling affinity ligands. Separopore® 4B provides good…

  • Aldehyde Separopore® (Agarose) 4B-CL

    bioWORLD

    Applications: Aldehyde Separopore® 4B has a robust reductive amino alkylation coupling chemistry to immobilize proteins and other ligands through primary amines (–NH2) to the resin. It can be used for a variety of affinity purification methods involving batch or column chromatography.…

  • CNBr-activated Separopore® Agarose 4B

    Cyanogen bromide-activated Separopore® 4B matrix is used for preparation of resins for affinity chromatography. Pre-activated medium is suitable for immobilization of ligands containing primary amines. Cyanogen bromide (CNBr) in base reacts with hydroxyl groups on Separopore® 4B to form cyanate…

  • Cu-Chelated Separopore® Agarose

    bioWORLD

    Cu-Chelated Separopore® is an imminodiacetate (IDA) immobilized metal affinity chromatography (IMAC) matrix that seperates proteins via their differences in affinity to Cu metal ions. Properties Affinity matrix for proteins. Imminodiacetate immobilized matrix charged with Cu metal ions.v…

  • CDI-Activated Separopore® Agarose 4B

    Pre-activated medium for immobilization of ligands containing primary amines N, N'-Carbonyl diimidazole (CDI) mediated activation of Separopore® 4B matrix to allow coupling of the primary amino ligands by a stable amide bond formation No charge is introduced into the matrix after the coupling…

  • Lactose Separopore® (Agarose)6B-CL

    a-Lactose-Separopore® 4B-CL is used in protein chromatography, affinity chromatography and carbohydrate matrices. a-Lactose-Separopore® 4B-CL has been used to study microneme proteins and host-parasite cell surface receptors. Lactose-Separopore® 4B-CL may be used to isolate lactose binding…

  • Ubiquitin Separopore® (Agarose)

    bioWORLD

    Applications: Ubiquitin-Separopore® 4B is useful for binding the ubiquitin activation enzyme (E1), ubiquitin carrier enzymes (E2s), ubiquitin ligases (E3s) and ubiquitin-terminal hydrolyases. Ubiquitin-Separopore® 4B is used in affinity chromatography to study enzymes involved in…

  • Anti-Bovine Albumin BSA Separopore®

    bioWORLD

    Application: Anti-BSA-Separopore® 4B is used for immunoprecipitation and immunoaffinity purification of bovine serum albumin (BSA) from solutions (and co-immunoprecipitation of biomolecules that bind BSA). It is also used to remove BSA from solutions.

  • Fucose Separopore® (Agarose) 4B-CL

    Fucose-Separopore® 4B-CL used as affinity matrix for the purification of fucose-binding glycoproteins. Fucose is linked to Separopore® 4B-CL via a spacer arm to ensure efficient binding of this ligand to the protein.

  • DEAE Separopore® (Agarose) 6B-CL

    DEAE-Separopore® 6B-CL is a weak anion exchanger with excellent flow capacities and a high capacity for binding and resolution of proteins of all pI values. bio-World's DEAE-Separopore® 6B-CL was prepared by covalent attachment of diethylaminoethyl (DEAE) to Separopore® 6B-CL. DEAE remains charged…

  • Papain Separopore® (Agarose) 4B

    Papain-Separopore® 4B can be used as substitute of free papain in many applications. Papain is a cysteine protease that cleaves peptide bonds of basic amino acids, leucine or glycine. It also hydrolyzes esters and amides. This matrix is used to produce Fab fragment of IgG antibodies. It is also…

  • Dextran Separopore® (Agarose)4B-CL

    Dextran is coupled to a high strength Separopore® 4B-CL matrix. Coupling dextran to this material expands the surface area of the Separopore® beads allowing for further binding of macromolecules. Dextran-Separopore® 4B-CL is used in affinity chromatography as a protein binding site. It has been…

  • KLH Separopore® (Agarose) 4B

    Affinity matrix with immobilized KLH used for the purification of antibodies generated against peptide-KLH conjugated antigens byremoving antibodies against the KLH portion of the antigen. Anti-KLH antibodies can be removed by solid phase immuno-affinity column chromatography over the KLH-…

  • Thiophilic Separopore® Agarose 4B-CL

    bioWORLD

    Applications: Thiophilic affinity purification is based on the ability of some proteins, particularly immunoglobulins, to bind to this immobilized ligand. The term "Thiophilic" refers to an affinity for sulfone groups that lie in close proximity to thioether groups. Thiophilic adsorption…

  • Separopore® (Agarose) 6B

    bioWORLD

    Applications: Separopore® 6B is a high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands. It is ideal for both analytical and preparative protein purifications. Separopore® 6B is a proven base matrix for coupling affinity ligands. Separopore® 6B…

  • IDA Chelating Separopore® (Agarose) 4B-CL

    Metal ion-chelating Separopore® 4B-CL is designed for the affinity purification of 6X Histidine-tagged proteins. Nickel, Cobalt, Copper and Zinc are used as cations for this type of purification because they provide effective binding and selectivity for a wide range of proteins. Affinity matrix for…

  • NADP-Separopore® (Agarose) 4B-CL (Lyophilized)

    NADP covalently bound to Separopore® has a high affinity for NADP-dependent enzymes and dehydrogenases. Acetyl-CoA binds to NADP-Separopore® 4B-CL twelve times higher than NAD-Separopore® 4B-CL. NADP-Separopore® 4B-CL has also been used to purify extracellular nucleases.

  • E. coli Protein-Separopore® (Agarose) 4B

    bio-World provides immobilized E. coli proteins on a solid bio-affinity support for the removal of antibodies to E. coliproteins from antisera prepared against recombinant proteins expressed in E. coli. Our immobilized E. coli lysate consists of partially purified proteins from E. coli strains…

  • Tosyl-activated Separopore® Agarose

    bioWORLD

    Applications: Tosyl-activated Separopore® 4B-CL is used to couple primary amines, thiols, imidazole or tyrosine hydroxyl groups to the matrix. Tosyl-activated Separopore® 4B-CL couples more rapidly and efficiently to sulfhydryl groups than to primary amines. Coupling of antibodies through…

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