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CDI-Activated Separopore® Agarose 4B
- Pre-activated medium for immobilization of ligands containing primary amines
- N, N'-Carbonyl diimidazole (CDI) mediated activation of Separopore® 4B matrix to allow coupling of the primary amino ligands by a stable amide bond formation
- No charge is introduced into the matrix after the coupling reaction
- No ligand leakage after coupling
- Superior stability: Activated matrix is much more resistant to hydrolysis
- Ideal for immobilization of large protein ligands such as antibodies as well as peptides and nucleic acids
- Coupling reaction can also be carried out in organic solvents
- High coupling efficiency and easy to handle
- CDI-activated Separopore® 4B is activated with CDI groups for covalent immobilization of proteins and other molecules that contain N-nucleophiles
Group to be coupled: | -NH2 groups |
Matrix: | Separopore® 4B (agarose beads, 4%) |
Particle size range: | 52 - 165 µm |
Molecular weight range: | 6 x 104 - 2 x 107 |
Coupling conditions: | pH: 8.5 – 11, Temp: 4 – 25 °C, Time: 6 – 24 h |
pH stability: | 11 (ligand dependent) |
Storage: | 2 - 8°C |
Supplied as suspension in acetone |
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Product Detail
- Description
- CDI-Activated Separopore® 4B, 5 mL
- Packaging Size
- 5 mL
- UN Number
- 41115700
list price/quantity | total | | $0.00 |
- Description
- CDI-Activated Separopore® 4B, 25 mL
- Packaging Size
- 25 mL
- UN Number
- 41115700
list price/quantity | total | | $0.00 |
- Description
- CDI-Activated Separopore® 4B, 100 mL
- Packaging Size
- 100 mL
- UN Number
- 41115700
list price/quantity | total | | $0.00 |
- Description
- CDI-Activated Separopore® 4B, 500 mL
- Packaging Size
- 500 mL
- UN Number
- 41115700
list price/quantity | total | | $0.00 |
Product is restricted and can only be purchased by customers with a web profile linked to a Thomas Business Account -
Click here to login or
create your web profile. If you do not have a Thomas Business Account, please
Contact Us and someone will respond with details on how to apply for a business account with us.