MEM, originally prepared by Harry Eagle, is one of the most popular cell culture media. Upon his attempts to cultivate normal mammalian fibroblasts and certain HeLa cell subtypes, it was revealed that the nutritional needs of these cell types could not be met by BME. Further studies led to the…

Tween 80 is a polyethylene sorbitol ester, non ionic surfactant and an emulsifier derived from polyethoxylated sorbitan and oleic acid. Tween 80 has been widely used in biochemical applications including: solubilizing proteins, isolating nuclei from cells in culture, growing of tubercule…

Heat Inactivated. This serum is collected from whole blood from healthy individuals.

Xylenes are aromatic hydrocarbons. Xylene is used as a solvent. It is used in the laboratory to make baths with dry ice to cool reaction vessels, and as a solvent to remove synthetic immersion oil from the microscope objective in light microscopy.

Polyethyleneimine is a branched chain polymer having a ratio of 1:2:1 of primary:secondary:tertiary amines with a branching site every 3 to 3.5 nitrogen atoms and a base structure of (CH₂CH₂NH)x. The nitrogen atoms must be protonated to achieve cationic charge, so the cationic…

Triton® X-100 is a alkylaryl polyether alcohol. It is a nonionic surfactant prepared by the reaction of octylphenol with ethylene oxide. The number ("-100") relates only indirectly to the number of ethylene oxide units in the structure. The principal uses of Triton® X-100 is…

Spray on and rinse off for an Rnase-free surface! Completely removes Rnase contamination from bench tops, glass and plasticware. Rnase Erase is nontoxic and comes packaged with an adjustable spray bottle for for ease of use. Applications RNase ERASE® reagent is a precisely formulated,…

Dulbecco's Modified Eagle's Medium (DMEM) is the most widely used modification of Eagle's Basal Medium (BME). DMEM contains four times greater concentration of amino acids, vitamins and supplementary components. The original formulation calls for 1000 mg/L of glucose, which was first…

Propylene glycol is a clear, colorless and hygroscopic liquid. Propylene glycol contains an asymmetrical carbon atom, so it exists in two stereoisomers. The freezing point of water is depressed when mixed with propylene glycol due to increased opportunity for hydrogen bonding. Propylene glycol…

2-Merceptoethanol is used to reduce disulfide bonds and can act as a biological antioxidant by scavenging hydroxyl radicals. 2-Merceptoethanol is used to reduce disulfide linkages in solubilizing proteins for gel electrophoresis. 2-mercaptoethanol is readily oxidized in air to a disulfide,…

Hydrogen peroxide is used as a reagent, disinfectant, antiseptic, and propellant. In synthetic chemistry, H2O2 is used for epoxidation and hydroxylation transformations, and is used as the raw oxidant source for generating more complicated and specific organic/inorganic peroxides.

SDS is an anionic detergent used to solubilize and denature protein for Protein Electrophoresis & Blotting. SDS has also been used in large-scale phenol extraction of RNA to promote the dissociation of protein from nucleic acids when extracting from biological material. Surfactant used for…

2-8 CELLsium is a cytoprotective, protein free, ready-to-use bio-solution without Dimethyl Sulfoxide (DMSO) for the short-term storage of cells or tissue. For short-term storage, add cold (2-8 °C) 2-8 CELLsium at a recommended cell concentration of 1 x 10 6 mL to 8 x 10 6 mL. The 2-8 CELLsium…

Isopropyl alcohol is widely used in chemistry, biochemistry and molecular biology. Industrial applications include its use in antifreezes, quick-drying oils, and quick-drying inks, and as a solvent for gums, shellac, and essential oils. Isopropyl alcohol has been utilized as a solvent in the…

Fetal Bovine Serum (FBS) is the growth supplement of choice for cell culture due to its high level of nutrients. Most cell types and cell culture systems will benefit from the inclusion of FBS in growth media. It is produced to the highest standards to ensure product performance and safety with…

Acetic anhydride is a reagent that is used in the manufacturing of acetyl compounds and cellulose acetates. It is also utilized as a solvent in the examination of wool fat, glycerol, fatty and volatile oils, and resins. In the synthesis of organic compounds, acetic anhydride is used in various…

Trichloroacetic acid is an acetic acid analogue commonly used to precipitate proteins, DNA and RNA. In the presence of SDS Trichloroacetic acid will precipitate proteins which can then be quantified by the Lowry method. The compound has also found uses as a decalcifier and fixative in microscopy,…

Percoll® is a medium for density gradient centrifugation of cells, viruses and subcellular particles. Percoll® is composed of colloidal silica coated with polyvinylpyrrolidone (PVP), which renders the material completely non-toxic and ideal for use with biological materials. Centrifugation…

DNA-Erase™ is a non-alkaline, non-corrosive and non-carcinogenic cleansing solution that is highly active against DNA contamination and is intended for use at PCR workstations for eliminating PCR artifacts from all laboratory surfaces, including benchtops, apparatus and pipettors. Presence of…

Dimethyl sulfoxide (DMSO) is a highly polar aprotic organic reagent that has exceptional solvent properties for organic and inorganic chemicals. Dimethyl Sulfoxide is ideal for use as a cryoprotectant. Also used in chemical reactions, in polymerase chain reactions (PCR) as a PCR cosolvent to…

Acetone is a commonly used material in the manufacture of various chemicals like methyl isobutyl ketone, mesityl oxide, diacetone alcohol, acetic acid (via the ketene process), iodoform, and bromoform. Other products where acetone is utilized in manufacture include rayon, photographic films, and…

Protexidase buffer is 500 mL of PBS, pH 7.2, containing 0.5% BSA, antimicrobial preservatives and proprietary stabilizing compounds. This product stabilizes high dilutions of HRP-antibody conjugates, up to 1:16,000 dilution and inhibits microbial growth in dilute conjugates. This protective…

IGEPAL™ CA-630 is a homologous series of octylphenoxypoly(ethyleneoxy)ethanols used for the solubilizing and isolation of membrane proteins. The Igepal CA surfactants are all derived from the same hydrophobic starting material, octylphenol. By increasing the amount of hydrophilic…

A mineral oil is any of various colorless, odorless, light mixtures of alkanes in the C15 to C40 range from a non-vegetable (mineral) source, particularly a distillate of petroleum.Most often, mineral oil is a liquid by-product of the distillation of petroleum to produce gasoline and other…

Liqui-Gel is a 40% (w/v) solution of Ultra Pure Acrylamide (38.96%) and bis-Acrylamide (1.04%) in specially-prepared deionized water. Final ratio is 37.5:1. Acrylamide decomposes in the presence of acids, bases, oxidizing agents, iron, and iron salts. It decomposes non-thermally to form…

Mineral Oil is a colorless, odorless mixture of alkanes from a mineral source. It is often used as an inert overlay to minimize evaporation in molecular biology applications, including PCR, but has many other applications in other research fields.

Hexane is used in laboratory to extract oil and grease contaminants from water and soil for analysis.

Polysorbate 20 is typically used as an emulsifying agent for the preparation of stable oil-in-water emulsions, particularly in pharmaceutical applications. It has been used in pre-extraction of membranes to remove peripheral proteins. It has been used as a blocking agent on nitrocellulose. …

2-Mercaptoethanol is a hybrid of ethylene glycol and 1,2-ethanedithiol. It is used to reduce disulfide bonds and can act as a biological antioxidant by scavenging hydroxyl radicals. It is widely used because the hydroxyl group confers solubility in water and lowers the volatility. Due to its…

Dimethyl sulfoxide is a dipolar, aprotic solvent. Has been shown to accelerate strand renaturation (1-10% concentration) and is believed to give the nucleic acid thermal stability against depurination. By heating the solid form to approximately 30°C, the product can be melted without harming…

DNA-OFF is a non-carcinogenic, non-corrosive and heat resistant ready to use solution.