Cibacron Blue 3GA-Separopore® 6B-CL is an agarose conjugate in saline suspension used to searate and purify a variety of proteins. The ligand Cibacron Blue F3G-A is covalently coupled to Separopore® via chlorotriazine ring. The structure of the blue dye makes it a very versatile tool for separating…

Pre-activated medium for immobilization of ligands containing primary amines N,N\'-Carbonyl diimidazole (CDI) mediated activation of Separopore® matrix to allow coupling of the primary amino ligands by a stable amide bond formation No charge is introduced into the matrix after the…

Pre-activated medium for immobilization of ligands containing primary amines N, N'-Carbonyl diimidazole (CDI) mediated activation of Separopore® 4B matrix to allow coupling of the primary amino ligands by a stable amide bond formation No charge is introduced into the matrix after the coupling…

Benzamidine Separopore® 6B is p-aminobenzamidine covalently attached to Separopore ® 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is an inhibitor of trypsin-like serine protease. Trypsin and trypsin-like serine proteases bind to Benzamidine Separopore ® 6B and…

Dextran is coupled to a high strength Separopore® 4B-CL matrix. Coupling dextran to this material expands the surface area of the Separopore® beads allowing for further binding of macromolecules. Dextran-Separopore® 4B-CL is used in affinity chromatography as a protein binding site. It has been…

Quantitative Analysis:  Protein extract from E. coli containing a plasmid that expresses a maltose-binding protein-β-galactosidase fusion protein (100,000 units) is passed over a 1 ml column at 4°C. The column is then washed with 10 column volumes of 20 mM Tris-HCl (pH 7.4), 0.2 M…

Applications: Reduced glutathione is covalently linked Separopore® 4B for use in affinity purification of glutathione-S-transferase (GST) and GST-fused proteins. This product provides a one step purification method and permits rapid, mild and highly selective purifications of proteins…

DEAE-Separopore® 6B-CL is a weak anion exchanger with excellent flow capacities and a high capacity for binding and resolution of proteins of all pI values. bio-World's DEAE-Separopore® 6B-CL was prepared by covalent attachment of diethylaminoethyl (DEAE) to Separopore® 6B-CL. DEAE remains charged…

Applications: The immobilized red dye will bind to a wide variety of NADP + - and NAD + -dependent enzymes, including many kinases, dehydrogenases and transferases. Carboxypeptidase G, interferon, plasminogen and plasminogen activator are examples of other proteins that can be purified on…

Application: Hydrophobic chromatorgraphy.

A dye-ligand affinity matrix consists of Reactive yellow-3 bound to cross-linked 4% agarose beads (Separopore® 4B-CL).

Separopore® 6B-CL is crosslinked high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands. It is ideal for both analytical and preparative protein purifications. Separopore® 6B-CL is a proven base matrix for coupling affinity ligands. Separopore® 6B-CL…

Application: Hydrophobic chromatorgraphy.

A dye-ligand affinity matrix for purification of albumin, enzymes (including NAD+ and NADP+), coagulation factors, interferons and related proteins. The ligand, Cibacron Blue F3G-A, is covalently coupled to Separopore® 4B-CL via chlorotriazine ring. Due to a broad spectrum of the affinity, this…

Applications: Separopore® 2B-CL is a cross-linked bioagarose medium for gel filtration and coupling of affinity ligands. Hydrophilic backbone of Separopore® 2B-CL results in low non-specific interactions and high protein recoveries. Separopore® 2B-CL provides an excellent separation of proteins…

Separopore® 4B-CL is crosslinked high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands suitable for analytical and preparative protein purifications. Separopore® 4B-CL is a proven base matrix for coupling affinity ligands. Separopore® 4B-CL provides…

Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Applications: Separopore® 2B is a high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands. It is ideal for for both analytical and preparative protein purifications. Separopore® 2B is a proven base matrix for coupling affinity ligands. Separopore® 2B provides a good…

Separopore® 4B is a high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands. It is ideal for both analytical and preparative protein purifications. Separopore® 4B is a proven base matrix for coupling affinity ligands. Separopore® 4B provides good…

Separopore® 6B (macrobeads) is a larger sized agarose macrobead matrix for gel filtration and coupling of affinity ligands. It is ideal for both analytical and preparative protein purifications. Larger volumes available at discounted prices (enquire).

Used for protein purification by batch/column and scale-up procedures. Glutathione Separopore ®  Super Flow (S F) has an exellent protein binding capacity. The good flow properties of the 92 μm (average size) highly cross-linked Separopore ® matrix makes an…

Applications: Phenyl-Separopore® 4B-CL is used for hydrophobic interaction chromatography to purify membrane proteins and other proteins with hydrophobic regions. This matrix separates proteins based on the basis of the different hydrophobicities of the proteins.

Glutathione Separopore® HR is a high resolution matrix highly cross-linked 38 µm agarose bead (6%) matrix.Glutathione Separopore® HR ensures the lowest sample dilution and best high-resolution separation.

Applications: Tosyl-activated Separopore® 4B-CL is used to couple primary amines, thiols, imidazole or tyrosine hydroxyl groups to the matrix. Tosyl-activated Separopore® 4B-CL couples more rapidly and efficiently to sulfhydryl groups than to primary amines. Coupling of antibodies through…

Separopore® 4B (macrobeads) is a larger sized agarose bead matrix for gel filtration and coupling of affinity ligands. It is ideal for both analytical and preparative protein purifications. Larger volumes available at discounted prices (enquire).

Applications: Separopore® 6B is a high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands. It is ideal for both analytical and preparative protein purifications. Separopore® 6B is a proven base matrix for coupling affinity ligands. Separopore® 6B…