Fungus-Derived Proteinase K Purified Recombinant is prepared at 20 mg/mL in 10 mM Tris-HCl, 1 mM calcium acetate, pH 7.5 containing 50% glycerol. This Molecular Biology Grade Proteinase K solution is extremely effective on native proteins and can be used to rapidly inactivate endogenous nucleases…

Donor Horse Serum is suitable for use in diagnostic assays, as a supplement in mycoplasma growth and assay media and for culturing hematopoietic stem cells and neuronal cells. This serum is processed from whole blood collected from a controlled herd of young horses. Each animal is routinely…

HFBA is a strong acid and an ion-pairing agent. The strong acidity of HFBA ensures that other acidic groups such as carboxylic acid moieties on biomolecules remain protonated, and thus the samples are able to interact with organic solvents in such processes as reverse phase chromatography. The…

The antibiotics penicillin and streptomycin are used to prevent bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria. This solution contains 17,000 - 21,000 units/mL of penicillin and 18.0 - 23.0 mg/mL of streptomycin. …

CELLect Gold is collected from a U.S.D.A. regulated and monitored abattoir by technicians who work "on-site" where the fetal blood is aseptically collected via cardiac puncture. This raw material is transported to the production facility where it undergoes sterilization via filtration…

IGEPAL™ CA-630 is a homologous series of octylphenoxypoly(ethyleneoxy)ethanols used for the solubilizing and isolation of membrane proteins. The Igepal CA surfactants are all derived from the same hydrophobic starting material, octylphenol. By increasing the amount of hydrophilic…

Polysorbate 20 is typically used as an emulsifying agent for the preparation of stable oil-in-water emulsions, particularly in pharmaceutical applications. It has been used in pre-extraction of membranes to remove peripheral proteins. It has been used as a blocking agent on nitrocellulose. …

Glycerol is a simple polyol compound which is a viscous liquid that is widely used in pharmaceutical formulations. It has three hydroxyl groups that are responsible for its solubility in water and its hygroscopic nature. It is a trihydroxy sugar alcohol that is an intermediate in carbohydrate and…

2,2'-Azino-bis-(3-ethylbenzthiazoline-6-sulfonic Acid) Solution is oxidized to a radical action showing absorption maxima at 820 nm, 734 nm, 650 nm and 405 nm, in the presence of hydrogen peroxide and HRP. The latter frequency demonstrates a significant molar extinction coefficient and is…

Acetone is a commonly used material in the manufacture of various chemicals like methyl isobutyl ketone, mesityl oxide, diacetone alcohol, acetic acid (via the ketene process), iodoform, and bromoform. Other products where acetone is utilized in manufacture include rayon, photographic films, and…

Stabilized Chromogen Solution - This alkaline phosphatase chromogen solution contains the two components in a stabilized solution which may be used as the final step in color development. The resulting product is a permanent, deep-purple stain. It enhances results in both ELISA and immunohistology…

Triton® X-100 is a alkylaryl polyether alcohol. It is a nonionic surfactant prepared by the reaction of octylphenol with ethylene oxide. The number ("-100") relates only indirectly to the number of ethylene oxide units in the structure. Triton X-100 is a non-ionic surfactant and…

Phenol red is an acid-base indicator. It is made by condensing two moles of phenol with one mole of o-sulfobenzoic acid anhydride. Phenol Red is used as a pH indicator in cell culture applications. A solution of phenol red will have a yellow color at a pH of 6.4 or below and a red color at a…

Rabbit serum is a component of blood obtained from a rabbit that contains blood plasma without fibrinogen or other clotting factors. Rabbit Serum Combine with BSA to use as a blocking serum in immunocytochemistry and immunohistochemistry. In immunohistochemistry, antibodies are used to detect…

Human pooled serum is used as a media component to neutralize cell free virus. It's used as a reagent in liposome binding assay and in preparation of sperm. This serum is obtained from pooled, normal clotted human blood.

Catalase, Fungal suspension from from Aspergillus niger is long-acting, extremely stable form of catalase that is active over a wide pH range. Composed of four protein subunits Each subunit contains a heme group bound to its active site Catalase is used for the removal of peroxides,…

Protein purification reagent Triethanolamine acts as an organic additive in the grinding of cement clinker and as a complexing agent for aluminum ions in aqueous solutions. It is used as an emulsifier and a surfactant. It finds application in common products like dishwashing liquids, general…

L-glutamine is an essential amino acid that is a crucial component of culture media that serves as a major energy source for cells in culture. L-glutamine is very stable as a dry powder and as a frozen solution. In liquid media or stock solutions, however, L-glutamine degrades relatively rapidly.…

To rehydrate the powdered medium, dissolve 29.5 grams in one liter of water. Dispense as desired. Sterilize in the autoclave for 15 minutes at 15 pounds pressure at 121°C (Catalog number 16821 is a pre-sterilized solution). For more rapid growth of pathogenic bacteria, dissolve 1 gram of agar…

Isoamy alcohol is routinely used in molecular biology, especially in the purification of DNA. It is widely used in conjunction with phenol and chloroform (25:24:1 phenol : chloroform : isoamyl alcohol) for the removal of proteins from the nucleic acid solutions by extraction. Isoamyl alcohol is…

A buffer solution of HEPES can be prepared by any of several methods. The free acid can be added to water, then titrated with approximately one-half mole equivalent of sodium hydroxide or potassium hydroxide to the desired pH. A simple mixing table for preparing 0.05 M HEPES/NaOH has been…

Tweens® are a series of nonionic surfactants derived from sorbitan esters. They are soluble or dispersible in water but differ widely in organic and oil solubilities. Used as oil-in-water emulsifiers in pharmaceuticals, cosmetics, cleaning compounds, etc. Tween® 80 has been widely used…

Hydrogen peroxide is used as a reagent, disinfectant, antiseptic, and propellant. In synthetic chemistry, H2O2 is used for epoxidation and hydroxylation transformations, and is used as the raw oxidant source for generating more complicated and specific organic/inorganic peroxides.

N,N,N',N'-Tetramethylethylenediamin is a tertiary amine base. N,N,N',N'-Tetramethylethylenediamine is used to catalyze the formation of free radicals from ammonium persulfate or riboflavin. The free radicals will cause acrylamide and bis-acrylamide to polymerize to form a gel…

Ammonium acetate is a chemical compound which is derived from the reaction of ammonia and acetic acid. Ammonium acetate is volatile at low pressures. Because of which it has been used to replace cell buffers with non-volatile salts, in preparing samples for mass spectrometry. It is used as a buffer…

Undecane is a liquid alkane hydrocarbon. Undecane may be used as an internal standard in gas chromatography when working with other hydrocarbons. Since the boiling point of undecane (196°C) is well known, it may be used as a comparison for retention times in a gas chromatograph for molecules…

Dimethyl sulfoxide (DMSO) is a highly polar aprotic organic reagent that has exceptional solvent properties for organic and inorganic chemicals. Dimethyl Sulfoxide is ideal for use as a cryoprotectant. Also used in chemical reactions, in polymerase chain reactions (PCR) as a PCR cosolvent to…

TRITON X-114 is used in biochemical applications to solubilize and separate proteins due to its low cloud point (23 °C). At temperatures above the cloud point, detergent solutions separate into aqueous and detergent-enriched phases. This makes Triton X-114 particularly useful in…

MP Biomedicals offers a 1000X sterile filtered solution for use with serum-free media. It includes selenium, copper, zinc, and iron, as well as ferric citrate for greater stability.

Tween 80 is a polyethylene sorbitol ester, non ionic surfactant and an emulsifier derived from polyethoxylated sorbitan and oleic acid. Tween 80 has been widely used in biochemical applications including: solubilizing proteins, isolating nuclei from cells in culture, growing of tubercule…

Lipoprotein- cholesterol is a mixture of lipoprotein cholesterol, phospholipids and fatty acids. For use as a serum-free or serum-reduced media nutrient supplement, calibration of cholesterol values, and enzymatic or non-enzymatic cholesterol determinations.

Glutaraldehyde is a bifunctional protein cross-linking reagent, reacting with NH2 groups to form Schiff's bases. Sporicidal agent. Glutaraldehyde reacts through cross-linking to impart water resistance to protein and polyhydroxy compounds. It is also a reducing agent for photochemicals. In…