N,N,N',N'-Tetramethylethylenediamin is a tertiary amine base.
N,N,N',N'-Tetramethylethylenediamine is used to catalyze the formation of free radicals from ammonium persulfate or riboflavin. The free radicals will cause acrylamide and bis-acrylamide to polymerize to form a gel matrix which can be used for sieving macromolecules such as nucleic acids and proteins. TEMED has been used in the coating for soft polymers, such as polyacrylamide, in capillaries for investigation by atom force microscopy. Microchips containing oligonucleotides and proteins immobilized within gel pads have been prepared using TEMED. It is also used used in the HPLC of small organic molecules.
Suggested use is 6-10 uL for 10 mL acrylamide gel solution (the less amount of TEMED used, the slower the polymerization).
Grade: Molecular biology reagent
Purity: ~99%
Suitability: Suitable for Polyacrylamide Gel Electrophoresis
Key Applications: Polyacrylamide gel formation catalyst
Application Areas: Nucleic Acid Electrophoresis; Nucleic Acid Sequencing
Product Type: Organics
Chemical Class: Polar
Density: 0.775 g/mL at 20°C(Lit.)
Boiling Point: 120-122°C (Lit.)
Melting Point: -55°C(Lit.)
Refractive Index: n20/D 1.4179 (20°C)(Lit.)
Presentation: Liquid
Format: Liquid
pH: 10 - 12 (0.5% aq soln)
Detection Method: FTIR
Moisture content: ≤2%
Solubility: Easily soluble in water (20 gm/mL-clear-cololess solution).
Storage & Handling: Store at Room Temperature (15-30°C), store under nitrogen