HEPES is a zwitterionic organic chemical buffering agent; one of the twelve Good's buffers. HEPES has been utilized extensively for research and commercial applications in the cell culture and tissue culture industries. HEPES is widely used in cell culture, largely because it is better at…

MP EcoChrom™ Alumina B, Activity I are good "all-round" adsorbents for laboratory use as well as for industrial use. They can be adjusted to lower activity by the addition of polar media, preferably water, but also higher alcohols, e.g. glycol or glycerol can be used. An oxide of…

Borax is a salt of boric acid which is used as a buffer. It has a co-complexing ability with other agents in water to form complex ions with various substances. It is also commonly utilized in chromatography and electrophoresis studies. Borax is typically used as a buffer component,…

Sucrose is a nonreducing disaccharide composed of glucose and fructose, linked via their anomeric carbons. It is obtained commercially from Sugarcane, sugar beet (Beta vulgaris), and other plants and used extensively as a food and a sweetener. Use to create sucrose gradients for purification of…

Sodium phosphate is a reagent with very high buffering capacity that is widely used in molecular biology, biochemistry, and chromatography. Sodium phosphate occurs in several forms: monobasic (NaH2PO4), dibasic (Na2HPO4), and tribasic (Na3PO4). Most neutral sodium phosphate buffer solutions consist…

Potassium phosphate is a reagent with high buffering capacity. It occurs in several forms: monobasic, dibasic and tribasic. Most pH neutral potassium phosphate buffer solutions consist of mixtures of the monobasic and dibasic forms to varying degrees, depending on the desired pH. Potassium…

Potassium chloride is a salt commonly used in the preparation of phosphate buffered saline and for the extraction and solubilization of proteins. It is a good potassium source. Also used in the hypotonic KCl-propidium iodide protocol for univariate analysis of metaphase chromosomes ; dissociation…

Tris have been useful as buffers in a wide variety of biological systems. It has been used as a starting material for polymers, oxazolones (with carboxylic acids) and oxazolidines (with aldehydes). It does not precipitate calcium salts and is of value in maintaining solubility of manganese salts.…

Molecular Biology Certified™ Synonyms: Luria Broth Base with Agar (Miller modification) L-Broth Agar is a nutritionally rich medium originally developed for growth and maintenance of recombinant E. coli strains. MP Biomedicals offers L-Broth Agar medium in an encapsulated format…

Calcium chloride is useful in preparing competent bacteria. Calcium chloride dihydrate was used in the preparation of calcium-alginate beads. It was used to compose the medium for Thermophilic Clostridia (MTC). It may be used for the concentration of virus vectors in a protocol.

Glycine is a non-chiral amino acid that can be synthesized in the body from the amino acid serine by Serine Hydroxymethyltransferase. Acts as an inhibitory neurotransmitter Shown to block NMDA-stimulated dopamine release in fetal mesencephalic cell cultures Increase NMDA-receptor…

Potassium phosphate is a reagent with high buffering capacity. It occurs in several forms: monobasic, dibasic, and tribasic (K3PO4). Most pH neutral potassium phosphate buffer solutions consist of mixtures of the monobasic and dibasic forms to varying degrees, depending on the desired pH. …

Ammonium sulfate is an inorganic salt. Ammonium Sulfate may be used for the precipitation or fractionation of proteins or for purification of antibodies. Useful for crystallographic analysis of nucleic acids and proteins. The use of ammonium sulfate in long PCR buffer, in PCR lysis solution, and…

MP EcoChrom™ Alumina A, activity Super I shows adsorbtion capacity approximately twice as high as that of MP EcoChrom™ Alumina A, Activity I. Their inital activity is kept in a very narrow range and their deactivation behavior is extremely constant. MP EcoChrom™ Alumina A,…

Tris have been useful as buffers in a wide variety of biological systems. It has been used as a starting material for polymers, oxazolones (with carboxylic acids) and oxazolidines (with aldehydes). It does not precipitate calcium salts and is of value in maintaining solubility of manganese salts.…

Prior to lyophilization, the product is filtered through a 0.2 µm filter. Albumin is used to solubilize lipids, and is also used as a blocking agent in Western blots or ELISA applications. Globulin free albumins are suitable for use in applications where no other proteins should be present…

Gellan gum is a water-soluble polysaccharide produced by Pseudomonas elodea, a bacterium. Gelrite, gellan gum, is recommended as a gelling agent for use in place of agar in microbiological media. Gellan gum is used in a wide variety of immobilization matricies. As a food additive, gellan gum is…

CIRCLEGROW® is used for growth of E.coli and extends plasmid production past log phase. Molecular Biology Certified™ Obtain rapid growth of E.coli High yields of plasmid DNA At the end of log phase, cells growing in Circlegrow begin to utilize a proprietary second…

Glycine is commonly used as a component in Tris-glycine and Tris-glycine-SDS running buffers for polyacrylamide gel electrophoresis, a component of Towbin's transfer buffer for Western blots, a buffer substance in cryoenzymology, in osmotic pressure maintenance in isoelectric focusing of…

Tris is an excellent biochemical and biological buffer for all applications where ultra high purity is required. The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per…

Glycine is a non-essential amino acid. It is the only amino acid with no asymmetric carbon and thus is not chiral. It is the major inhibitory neurotransmitter. It is involved in the biosynthesis of the porphyrin rings of hemes and chlorophylls. Glycine is commonly used in buffer solutions, in…

Potassium bicarbonate is a widely used reagent in research and industrial applications. Potassium bicarbonate has been used in studies of renal disorders and the relationship of muscle injury to this process. Potassium bicarbonate has been shown to inhibit the growth of Aspergillus parasiticus in…

D(+)Glucose, Anhydrous is a granular monosaccharide. Glucose is a main source of energy for living organisms. Glucose occurs naturally in the free state in fruits and other parts of plants. Glucose is combined into glucosides, disaccharides, oligosaccharides, the polysaccharides (cellulose and…

Boric acid has a single boron; its salt forms complex equilibria in solution, with the ion B4O7-2 being the predominant species. Boric acid is used for weatherproofing wood and fireproofing fabrics; as a preservative; manufacturing of cements, crockery, porcelain, enamels, glass, borates,…

Sodium bicarbonate is broadly used as a physiological buffer in cell culture applications. The pKa for bicarbonate <-> carbonic acid reaction is 6.4 The pKa for bicarbonate <-> carbonate reaction is 10.3. Sodum bicarbonate has two pKas, 6.4 and 10.3. The compound can react with both…

Sodium metabisulfite is an antioxidant reducing agent, with antimicrobial and antifungal properties. Gastric tissue studies report that sodium metabisulfite can augment lipid peroxidation and apoptosis in a dose-dependent manner. Sodium metabisulfite is used as an active ingredient in campden…

Fructose, or fruit sugar, is a simple monosaccharide found in many plants. It is one of the three dietary monosaccharides, along with glucose and galactose, that are absorbed directly into the bloodstream during digestion. Fructose is a 6-carbon polyhydroxyketone. It is an isomer of glucose which…

Alanine is a nonessential amino acid that plays a key role in the glucose-alanine cycle between muscle tissue and the liver. In amino acid-degrading tissues such as muscle, amino groups are pooled as glutamate by transamination reactions. Used as an amino donor in conversion of…

Mannitol is carbohydrate found in plants and plant exudates. D-Mannitol is used with boric acid in the manufacturing of dry electrolytic condensers for radio applications. In pharmacy, used as as excipient and diluent for solids and liquids. In analytical chemistry for boron determinations. In…

Sodium chloride is a commonly used chemical which is found widely in nature. It is considered to be an essential nutrient. Excess amounts of sodium chloride can destroy electrolyte balance and cause death in most animals, including humans. Sodium chloride is used in a wide variety of biochemical…

Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle. Urea is a mild agent usually used in the solubilization and denaturation of proteins. It is also useful for renaturing proteins from samples already denatured…

Sucrose is a disaccharide composed of glucose and fructose. Sucrose can be used to prepare density gradients for cell/organelle separation. It is used as a supplement in plant, insect, and bacterial culture media. It can also be used in various enzymatic assays.