Sodium bicarbonate is broadly used as a physiological buffer in cell culture applications. The pKa for bicarbonate <-> carbonic acid reaction is 6.4 The pKa for bicarbonate <-> carbonate reaction is 10.3. Sodum bicarbonate has two pKas, 6.4 and 10.3. The compound can react with both acids and bases, therefore it can be used as a neutralizer. Sodium bicarbonate may be used to remove or wash acidic impurities from a crude liquid to yield a purer sample. Sodium bicarbonate reacts with the carboxyl groups to give a fast-forming and effervescent CO2 formation which has been used to test for the presence of carboxylic groups in proteins.
Purity: ≥99.5%
Key Applications: Biological Buffers, Buffers, Cell Biology, Cell Culture, Cell Signaling, Laboratory pH neutralizer that reacts with acids and bases
Product Type: Inorganics
Chemical Class: Salts
Density: 2.159 at 20°C(Lit.)
Melting Point: 270°C; decomposition begins at 50°C(Lit.)
Refractive Index: 1.500(Lit.)
Presentation: White Powder
Format: Powder
pH: 8.1 - 8.5 (0.1 M aq soln)
pKa: (1) 6.37, (2) 10.25 (carbonic acid)(Lit.)
Solubility: Soluble in water (100 mg/mL).
Storage & Handling: Store at Room Temperature (15-30°C)