Lonza Life Science Reagents
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DNA Marker/Ladder
LonzaLonza’s standard DNA Ladders are ready-to-dilute prior to loading on your gel. Plasmid-free to ensure minimal background. For all of Lonza’s Ladders, each band has an ideal 50% G-C content for rapid, precise sizing and sharp resolution. Our DNA Ladders are free from contaminating…
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Reliant™ Gels
LonzaThe Reliant™ Gel System consists of precast agarose minigels for rapid and reproducible resolution of DNA fragments from 8 bp to 10 kb. Each precast agarose gel is precision cast using our SeaKem® Gold, NuSieve™ Hi-Res or NuSieve™ 3:1 Agarose and are available…
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AccuGENE™ Water
LonzaAccuGENE™ Water is considered a “fit for purpose” Type I water and is created for use specifically in molecular biology applications.
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SeaKem® ME Agarose
LonzaSeaKem® ME Agarose is a moderate EEO agarose that is often used for serum protein electrophoresis, IEP and occasionally for nucleic acid electrophoresis. With a standard melting temperature and gel strength similar to SeaKem® LE Agarose, SeaKem® ME Agarose is optimized to quickly and…
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SYBR® Green stains are fluorescent dyes used to detect DNA and RNA. They are more sensitive and less mutagenic than standard stains, yet they provide excellent signal-to-noise ratio with minimal background. SYBR® Green I detects as little as 60 pg dsDNA and 1 ng oligonucleotides in agarose…
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SeaPlaque™ GTG™ Agarose is a Genetic Technology Grade™ Agarose used for separating DNA and PCR product fragments greater than 1,000 bp. This low melting temperature (65º C) agarose is ideally suited for direct enzymatic manipulation of nucleic acids in remelted agarose…
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Formaldehyde denaturation is suitable when samples are to be recovered from agarose gels. Our Formaldehyde Sample Buffer contains formaldehyde, formamide, and tracking dyes in MOPS Buffer. Supplied in 1.0 ml vials
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FlashGel DNA
LonzaFlashGelTM DNA Cassettes are essential components of the FlashGelTM System for fast electrophoresis of DNA. FlashGelTM DNA Cassettes are the ideal sample screening tool for checking up to 32 PCR or restriction fragment samples quickly without having to plan your day around agarose gels or to verify…
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SeaKem® Gold Agarose
LonzaSeaKem® Gold Agarose is a very high gel strength, low EEO, standard gelling temperature agarose. This Genetic Technology GradeTM Agarose is for rapid resolution of megabase DNA by pulsed field gel electrophoresis (PFGE). Due to its low EEO, the electrophoretic mobility of DNA in SeaKem®…
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AccuGENETM 1X PBS
LonzaAccuGENE™Phosphate buffered saline is a buffer solution commonly used in biological research. It is a water-based salt solution containing disodium hydrogen phosphate and sodium chloride. The osmolarity and ion concentrations of PBS are isotonic, so it is often used for protein and cell…
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AccuGENETM 10% SDS
LonzaAccuGENE™ Sodium dodecyl sulfate is a detergent that is known to denature proteins; It’s used in polyacrylamide gel electrophoresis for the determination of protein molecular weight. It is also used in nucleic acid extraction procedures for the disruption of cell walls and dissociation…
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Reliant™ RNA Gels 1.25%
LonzaReliant™ Precast RNA Gels are ready-to-use RNase free agarose gels suitable for a variety of RNA applications including Northern blotting and analysis of RNA transcripts. Gels are made with 1.25% SeaKem® Gold Agarose in 1X MOPS buffer with no denaturants. Reliant™ Precast…
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RNA Marker
LonzaRNA markers consist of RNA transcripts from 0.5 to 9 kb, in sizes of 0.5, 1, 1.5, 2, 2.5, 3, 4, 5, 6, and 9 kb. These RNA standards may be denatured with standard procedures, and can be visualized on Northern blots with labeled lambda sequence. Markers are provided at a concentration of 1 mg/ml.…
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AccuGENE™ Saline-sodium-citrate is used as a hybridization buffer, to control stringency for washing steps in protocols for Southern blotting, in situ hybridization or Northern blotting. 20X SSC may be used to prevent drying of agarose gels during a vacuum transfer.
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Checking Sample Quality with the FlashGel TM System for RNA – Sample degradation is visible at low levels on the FlashGel TM System for RNA. FlashGel TM RNA Cassette run for 8 minutes at 225 V, followed by 20 minute hold prior to imaging. Lane 1: RNA marker (Lonza); Lane 2: 250 ng E. coli…
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SeaKem® HGT Agarose
LonzaSeaKem® HGT Agarose is a high gelling temperature agarose that exhibits high gel strength and clarity and is primarily used the electrophoreisis of proteins. Specifically designed for applications such as counter-immunoelectrophoresis and crossed-immunoelectrophoresis, SeaKem® HGT Agarose…
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SeaPrepTM Agarose, 25 g
LonzaSeaPrepTMAgarose is a specialty 'soft agarose' product with ultralow gelling and melting temperature properties that make it valuable for many techniques involving biomolecules. SeaPrepTMAgarose is an excellent medium for hybridoma cloning and produces soft, clear gels. SeaPrepTM Agarose…
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SYPRO® Ruby Blot Stain
LonzaSYPRO® Ruby is 60-times more sensitive than reversible protein blot stains like Ponceau S, and 30-times more sensitive than Amido Black or Coomassie® brilliant blue stains.
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PCR Products on a NuSieve™ 3:1 Agarose Gel – A 550 bp sequence from lambda DNA was amplified (25 cycles) using primers and Taq DNA polymerase supplied in the GeneAmp® kit (Roche Molecular Systems). PCR products and controls were electro phoresed on a 4% NuSieve™ 3:1 agarose…
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The ProSieve™ QuadColor™ Protein Marker is a mixture of 12 recombinant, highly purified proteins with molecular weights of 4, 6, 10, 15, 25, 40, 55, 70, 100, 140, 170, 250, and 300 kDa. the proteins are individually prestained using four different dyes, producing a brightly colored…
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SeaKem HE AGAROSE
LonzaOne of Lonza’s highest EEO argaroses, SeaKem® HEEO Agarose is a very high EEO agarose that is useful in applications requiring significant cathodal migration, such as IEP of IgG and IgM. SeaKem® HEEO Agarose can also be used to blend with a lower EEO agarose to achieve a specific EEO…
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SYBR® Green II Stain
LonzaSYBR® Green Stains are fluorescent dyes used to detect DNA and RNA. They are more sensitive and less mutagenic than standard stains, yet they provide excellent signal-to-noise ratio with minimal background. SYBR® Green I detects as little as 60 pg dsDNA and 1ng oligonucleotides in agarose…
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MetaPhor™ Agarose
LonzaDNA ladders with 4 bp or 8 bp step sizes were prepared by ligation of Bgl II linkers. Aliquots of 0.8 µgof the ladders were separated on a 3.5% MetaPhor™ Agarose gel in a horizontal format and compared to an 8% polyacrylamide gel run in a vertical format in TBE buffer.
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FlashGel System
LonzaThe FlashGel™ System (Cat. No. 57067) contains a FlashGel™ Dock, a FlashGel™ Camera, a pack of 1.2% FlashGel™ DNA Cassettes, Loading dye and Marker.
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SYPRO® Ruby Gel Stain
LonzaSYPRO® Ruby Protein Gel Stain is highly sensitive, simple to use fluorescent stain that can accurately quantitate protein expression levels, and is compatible with standard fluorescent visualization systems and downstream identification techniques such as mass spectrometry.