Checking Sample Quality with the FlashGelTM System for RNA – Sample degradation is visible at low levels on the FlashGelTM System for RNA. FlashGelTM RNA Cassette run for 8 minutes at 225 V, followed by 20 minute hold prior to imaging. Lane 1: RNA marker (Lonza); Lane 2: 250 ng E. coli Total RNA; Lanes 3 – 7: E. coli Total RNA incubated with increasing levels of RNase A. Intact, denatured RNA shows sharp, clear bands on the FlashGelTM System. Partially degraded RNA has a smeared appearance, and completely degraded RNA appears as a low molecular weight smear.
The FlashGelTM System for RNA Starter Pack is optimized for the unique requirements of RNA, and is a fast and sensitive tool for analysis of sample integrity. The system is recommended for verification and analysis of total RNA, quick checks of native RNA, and checking RNA degradation and mRNA purity. RNA fragments are analyzed on The FlashGelTM System using the same fast and simple procedure used for DNA. Separation is complete in <8 minutes and RNA samples are ready for imaging within 10-20 minutes. The FlashGelTM System for RNA is 5-20X more sensitive than ethidium bromide gels. RNA <10 ng per band are clearly detected, conserving precious RNA samples FlashGelTM RNA Cassettes fully enclose the gel, stain and running buffer, protecting samples from contaminating RNases. RNA cassettes are designed for performance and purity, and are guaranteed RNase free. The FlashGelTM Dock provides electrophoresis and visualization of both DNA and RNA cassettes.
Benefits:
- Get results in 30 minutes or less
- Detect <10 ng RNA per band
- Avoid hazardous reagents and contiminating RNases
Applications:
Content & Storage:
- FlashGelTM RNA Cassettes, 1.2% 12+1 well, single-tier, 9 pack
- Formaldehyde Sample Buffer (with bromophenol blue and xylene cyanol)
- RNA Marker 0.5 bp – 9 kb
- Molecular Biology Grade Water