Agarose II has a low melting and gelling temperature compared to standard agaroses. The low melting temperature allows for the recovery of undamaged nucleic acids below the denaturation temperature. The low gelling temperature ensures that the agarose will be in a liquid state at a temperature range where in-gel manipulations can be performed without prior extraction from the agarose slice.
Specifications
- Moisture: ≤ 7%
- Ash: ≤ 0.4%
- EEO (electroendosmosis): ≤ 0.12
- Sulfate: ≤ 0.12%
- Clarity 1.5% (NTU): ≤ 4
- Gel Strength 1.5% (g/cm2): ≥ 500
- Gelling Temperature 1.5% (ºC): 24-28
- Melting Temperature 1.5% (ºC): ≤ 65.5
- DNase/ RNase Activity: None Detected
- DNA Resolution ≥1000bp: Finely resolved
- Gel background: Very low
Appropriate Agarose Concentrations
| GEL CONCENTRATION (%) | 1X TAE BUFFER | 1X TBE BUFFER |
| 0.75 | 500-20,000bp | 500-12,000bp |
| 1.00 | 300-16,000bp | 300-8,000bp |
| 1.25 | 250-10,000bp | 200-4,000bp |
| 1.50 | 200-5,000bp | 150-3,000bp |
| 1.75 | 100-2,500bp | 100-2,000bp |
| 2.0 | 50-1,500bp | 50-1,000bp |
Features
- Lower gel strength compared to standard agarose
- Higher clarity
- Easy to handle
Applications
- Electrophoresis of DNA Fragments >1000bp
- Tissue and Cell Culture
- Viral Plaque Assays