The potency of an antibiotic can be determined by chemical, physical and biological means. An assay is made to determine the ability of an antibiotic to kill or inhibit the growth of living microorganisms. Biological tests offer the most convenient means of performing an assay (1), since a…

Bile Salts is extracted under controlled conditions from purified fresh bile, to be used in bacteriological culture media as a selective inhibitory agent for isolation and identification of pathogens. It is used in media for example MacConkey Agar/Broth, SS Agar, VRBA, Fluid Tetrathionate Medium,…

McFeters, Cameron and LeChevallier modified Tergitol 7 Agar to improve its selective and differential properties for the recovery of stressed coliforms from chlorinated water (1). They have reported that the selective media such as M-Endo Agars used to isolate gram-negative bacteria recovered only…

Casman Broth Base is used for isolation of fastidious microorganisms from clinical specimens under reduced oxygen tension. Proteose peptone, tryptose and HM Peptone B provide amino acids and other complex nitrogenous nutrients. Dextrose improves growth of pathogenic cocci. Corn starch prevents…

Fungi Kimmig HiVeg™ Agar Base is recommended for cultivation, isolation, identification and preservation of fungal strains. Hiveg media promotes the development of growth forms, which are used as important characteristic criteria for identification, used for the cultivation, identification and…

This medium is also known as cystine glucose blood agar and is the most suitable medium for isolating F.tularensis. Haemoglobin provides additional nutrients and growth factors. This medium also supports growth of gram-negative cocci and other pathogenic microorganisms without additional…

Hiveg medium Recommended for the selective enrichment of Salmonella including Salmonella Typhi from faeces, urine, food and other material of sanitary importance. The medium, originally formulated by Mueller is recommended by APHA for enrichment of Salmonella. Due to the addition of iodine and…

Liver Extract Powder is a dehydrated extract of fresh liver tissues of healthy animals. It is processed under controlled conditions to retain all the nutritive values of liver. Appearance Brownish yellow to brown Homogenous Free flowing powder, having Characteristic odor but not putrescent. …

Dubos and Middlebrook (1) developed various formulations containing oleic acid and albumin, which protect Mycobacterium from toxic agents, helping for the growth of tubercle bacilli. Middlebrook 7H10 Agar Base was formulated as per Middlebrook, Cohn et al (2) reformed the original oleic…

Casein Acid Hydrolysate is used in preparation of microbiological media. It is an acid hydrolysate of casein. It has Sodium chloride less than 3% and is Vitamin free. Appearance Off white to light yellow Homogenous Free flowing powder, having Characteristic odor but not putrescent. …

MUG EC HiVeg™ Broth is recommended for detection of Escherichia coli in water and food samples by a fluorogenic method. Hiveg media recommended by APHA for the analysis of drinking water, surface and ground water and waste-water for the presence of Escherichia coli. It permits rapid detection of E.…

The coliform group consists of several genera of bacteria belonging to the family Enterobacteriaceae . The historical definition of this group has been based on the method used for detection i.e. lactose fermentation. This group is defined as all aerobic and facultative anaerobic, gram-negative,…

Fraser HiVeg™ broth base is recommended as a primary as well as secondary enrichment medium, for the isolation and enumeration of Listeria monocytogenes from food and animal feeds. Hiveg media used for the detection of Listeria species in food products. It provides optimum conditions for the growth…

KG agar base is recommended for promoting fast and free sporulation to distinguish between Bacillus cereus and Bacillus thuringiensis. It is also recommended by APHA. Peptone and yeast extract in the medium supports the growth of B.cereus, B.thuringiensis. Lecithinase activity is observed as an…

This media is formulated as per American Public Health Association for presumptive identification and confirmation of coliform bacteria. During examination of water samples, growth from presumptive positive tubes showing gas in Lactose Broth (M026) or Lauryl Tryptose Broth (M080) is inoculated in…

HM Peptone is used for routine and mass scale cultivation of organisms for production of antibiotics, enzymes, and vitamin production. It is also use for general purpose culture media for routine cultivation of a variety of microorganisms. It is equivalent to Meat Peptone. Application: For Routine…

It is used for studies of haemolytic activity of Clostridia, Streptococci and other anaerobes. For isolation or cultivation of the highly fermentative butyric types, 1% dextrose may be added prior to sterilization. Application: For the Isolation & Identification of Anaerobic Pathogens & For…

Clostridium perfringens food poisoning is one of the most common types of human food borne illness (1). The foods usually involved are cooked meat or poultry products containing large numbers of viable cells. A heat- labile enterotoxin produced only by sporulating cells (2) induces the major…

Peptonized Milk is an enzymic digest of high grade skimmed milk powder. It has high tryptophan content and is therefore used in media for testing the indole reaction. It serves as a source of nitrogen and also has high level of carbohydrate. Suitable for cultivation of Lactobacilli, yeasts and…

WL Differential HiVeg™ Broth for selective isolation and enumeration of bacteria encountered in breweries and industrial fermentations containing mixed flora of yeast and bacteria. Bakers yeast counts can be carried out in this medium at a pH 5.5. By adjusting the pH to 6.5, the medium can be used…

Thermophillic bacteria are usually species of Bacillus which enter milk from various sources on the farm or from poorly cleaned equipments in the processing plant. These bacteria rapidly increase in numbers when present in milk or dairy products that are held at high temperature for long periods.…

Recommended for the primary isolation and subsequent cultivation of the tubercle bacilli. Application: For Laboratory Chemicals, Analytical Purpose, Biochemical Analysis for Invitro Diagnostic Use Appearance: Light yellow to brownish yellow homogeneous free flowing powder Gelling: Firm,…

Peptone Water is particularly suitable as a substrate in the study of indole production. Peptic digest of animal tissue used in Peptone Water is rich in tryptophan content. Presence of indole can be demonstrated using either Kovacs or Ehlrich reagent. Peptone Water is also utilized as a base for…

Acid Broth is recommended for the cultivation of acid tolerant microorganisms from canned food. It is a good medium for the recovery of minimal contamination of canned acid food and is formulated as per APHA for the selective cultivation of acid tolerant microorganisms from canned foods. It…

Granulated Hektoen Enteric Agar is for differential and selective isolation of Salmonella and Shigella species from enteric pathological specimens.

Recommended for the microbiological assay of Amphotericin B and Nystatin using Saccharomyces cerevisiae. The medium is prepared as per the formula of Grove and Randall Medium 12. Appearance: Cream to yellow homogeneous free flowing powder Gelling: firm, comparable with 2.5% agar gel Colour and…

Brain Heart Infusion Medium is useful for cultivating a wide variety of microorganisms since it is a highly nutritive medium. Brain Heart Infusion Broth is a modification of the original formulation of Rosenow, where he added pieces of brain tissues to dextrose broth (1). Brain Heart Infusion Broth…