Recommended for the microbiological assay of Amphotericin B and Nystatin using Saccharomyces cerevisiae. The medium is prepared as per the formula of Grove and Randall Medium 12.
- Appearance: Cream to yellow homogeneous free flowing powder
- Gelling: firm, comparable with 2.5% agar gel
- Colour and clarity of prepared medium: yellow coloured clear to slightly opalescent gel forms in petri plates
- Reaction of 6.25% w/v aqueous solution at 25°C. pH : 6.1±0.2
- Cultural characteristics observed after an incubation at 25-30°C for 18-24 hours