Size Exclusion Chromatography or gel filtration is a widely used technique for separation of proteins and other molecules based on their sizes. G-Sep™ Size Exclusion Columns are composed of high performance rigid resin made up of co-polymer of agarose and dextran. The Columns are available…

We offer a range of secondary antibodies conjugated to either alkaline phosphatase (AP) or horseradish peroxidase (HRP). The antibodies are isolated from antisera by immuno-affinity chromatography using antigen coupled to sepharose beads or other methods. They are supplied lyophilized, from a…

An important tool in research is the generation of peptide and protein affinity columns for the purification of antibodies and for the discovery of important interacting proteins and cofactors. G-Biosciences offersa complete kit for the coupling of peptides and proteins to agarose through their…

Our Bacterial Gram Staining kit is supplied with three strains of bacteria and all the necessary prealiquoted components to carry out the Gram staining to determine Gram positive and negative bacteria. The Gram staining method was first described in 1844 by the Danish bacteriologist Hans…

G-Trap™ SP Agarose Fast Flow is ready to use prepacked column used for seperation of proteins and other biomolecules based on charge. The resin in G-Trap™ SP Agarose Fast Flow is a strongcation exchanger composed of highly crosslinked 6% agarose beads coupled with sulphopropyl (SP)…

Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1975), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals. The Co-NTA Resin is specifically designed for the purification of…

The Column-PROTEIN-Concentrate™ kit has been specifically developed for concentration of those proteins that cannot be concentrated either by precipitation or other techniques. The kit is based on a proprietary Protein Binding Resin that binds and immobilizes any protein in a low salt buffer…

The Quantitative Precipitin Technique is a simple technique that is routinely used in the analysis of antibody and antigen interactions and for the estimation of the antibody or antigen content in a sample. The technique is based on the interaction of antibody and antigen to form a large protein…

A selection of genomic isolation kits are offered that purify high quality genomic DNAfrom a wide variety of sources and for a wide array of applications. Our OmniPrep™ genomic DNA kits are for ultra pure genomic DNA that is suitable for all downstream applications. These kits are fully…

This kit is designed to teach 8 groups of four students the principles of Protein Electrophoresis. The kit contains the reagents to allow students to: Conduct a serial dilution of a stock amylase solution Prepare samples for PAGE electrophoresis Separate and distinguish proteins by…

The SpinOUT™ PCR columns are spin format desalting columns that have the ability to remove salts, radioisotpes, dyes, primers and deoxynucleotides (dNTPs) for nucleic acids following PCR. Two sizes are available: SpinOUT™ PCR-20 :For rapid purification of PCR products (>100…

The premade blots are prepared with the appropriate GenLysate and 50μg protein is loaded onto a 4-20% denaturing polyacrylamide gel, along with a prestained molecular weight marker.  After the proteins are resolved they are transferred to a PVDF membrane, using Efficient Western Transfer…

This kit is designed to teach 8 groups of four students the principles of Ion Exchange Chromatography and protein purification.  The kit contains the reagents to allow students to: Equilibrate a DEAE agarose column Load a protein sample on the column (amylase, lysozyme or a mixture of…

HOOK™ 6X His Protein Purification kit allows for the purification of soluble, 6X His tagged protein from bacterial cultures.The bacteria are first lysed with Bacterial PE LB™ and PE LB™-Lysozyme to release total soluble protein, whilst maintaining the structure and activity…

The premade blots are prepared with the appropriate GenLysate and 50μg protein is loaded onto a 4-20% denaturing polyacrylamide gel, along with a prestained molecular weight marker. After the proteins are resolved they are transferred to a PVDF membrane, using Efficient Western Transfer…

HOOK™-PEG₂-Iodoacetyl-Biotin reacts with protein free sulhydryl groups. Sulfhydryl reactive reagents are more specific and react only with free sulfhydryl residues (-SH or thiol groups). The side chain of the amino acid cysteine is the most common source of free sulfhydryl groups.…

Microorganisms, such as bacteria and fungi, are all around us. This kit allows students to collect samples from the world around them and grow the microorganisms so that students can visualize the microorganisms.  This teaches students how to plate and grow microorganisms.   …

G-Biosciences Silica Magnetic Beads are Fe₃O₄ magnetic beads coated with a silicon dioxide (SiO₂) layer. Since silica is able to bind to the nucleic acids, G-Biosciences Silica Magnetic Beads serve as a simple and efficient tool for plasmid DNA purification for…

Prepacked purification columns for purification are available in packs of 5.The columns available are the anionic chromatography columns, hydrophobic columns, size exclusion (gel filtration) columns.

Bacterial plasmids, the non-genomic transferable DNA, can easily be purified from bacteria using numerous techniques.  The purification of DNA is important for genetic research as it provides a source of transferable DNA and allows researchers to isolate large amounts of recombinant DNA. …

G-Trap™ FPLC Columns are empty columns designed for fast protein liquid chromatography. G-Trap™ FPLC Columns are made of polypropylene and come in 1 ml and 5 ml sizes. Features: Column material:  polypropylene   (PP) Frit material:  Ultra high…

G-Trap™ GT-1200 Desalting Column is prepacked ready to use column for separation of low molecular weight substances from high molecular weight substances based on size exclusion chromatography. G-Trap™ GT-1200 Desalting Columns are mostly used for removal of salt or buffer exchange…

Cetyltrimethylammonium bromide extraction solution for genomic DNA extraction. Applications Genomic DNA extraction buffer solution.

The XIT ™ Genomic DNA from Plant Tissue kit is designed for the isolation of genomic DNA from fresh or frozen plant tissues. The kit uses three main steps (see figure) that are rapid & efficient cell lysis, enzymatic protein digestionand complete protein precipitation, followed by…

This kit is designed to teach 8 groups of four students the principles of Western blotting. The kit contains the reagents to allow students to: Conduct a serial dilution of a stock amylase solution Prepare samples for PAGE electrophoresis Separate proteins by electrophoresis …

Thiophilic adsorption or thiophilic chromatography is a routinely used technique for the low cost, simple purification of immunoglobulins. Thiophilic adsorption was first developed by Porath et al 1 in 1984 and is a group specific, salt-dependent purification technique that has distinct affinity…

A comprehensive program that prepares adults and teenagers for a variety of post-secondary options including community or career college biotechnology certificate programs, four-year biotechnology degree programs, and industry workplaces. The vast text and laboratory curriculum was development…

Extracts and solubilizes nearly all of the proteins from mammalian samples, including membrane as well as soluble proteins, by a strong chaotropic extraction buffer to solubilize even the most difficult proteins. Suitable for biological samples from animal tissues and adherent and suspension cells.…

G-Sep™ Phenyl Agarose Fast Flow (FF) is a 6% cross-linked agarose with phenyl groups for Hydrophobic Interaction Chromatography (HIC). The resin is used to separate biomolecules on the basis of relative hydrophobicity. G-Sep™ Phenyl Agarose Fast Flow is available in two levels of…

G-Biosciences CDI Amine Reactive Agarose consists of 6% cross-linked agarose activated with CDI (1,1’-carbonyl diimidazole) to form reactive imidazole carbamates. The activation of the resin occurs in solvent and to maintain its activity the resin is supplied in acetone to prevent…

G-Biosciences’ Carboxyl Magnetic Beads are 1µm, uniform magnetic beads with surface functional group -COOH. The magnetic bead consists of a single-crystal Fe₃O₄ sphere core and dextran coating layer. Through chemical modification of dextran, the carboxyl…

The XIT ™ Genomic DNA from Mouse Tail kit is designed for the isolation of genomic DNA from 5mm sections of mouse tail. The kit uses three main steps (see figure) that are rapid & efficient cell lysis withenzymatic protein digestionand complete protein precipitation, followed by DNA…